21209-1-AP

antibody from Proteintech Group

Targeting: CCDC25 FLJ10853

Provider product page for 21209-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [18]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 21209-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21209-1-AP, RRID:AB_10732690
Product name: CCDC25 antibody
Antibody type: Polyclonal
Description: KD/KO validated CCDC25 antibody (Cat. #21209-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CCDC25 protein structure - 21209-1-AP shown in red.

Submitted references Chrysophanein of Rhubarb rescues ILC3-Derived IL-22 by blocking CCDC25/ILK/HIF-1α for mucosal healing in ulcerative colitis mice.

Xu B, Cao S, Li C, Zhao M, Wang Q, Xie X, Chen H, Xu R, Yi Q, Ke X, Zhu Y, Zhou L, Luo X
Journal of ethnopharmacology 2026 Jan 30;355(Pt A):120628

Tumor-Derived Alpha-1 Antitrypsin Promotes Liver Metastasis of Colorectal Cancer Through the Neutrophil Extracellular Traps-CCDC25 Pathway.

Fei Q, Li C, Zhan L, Li X, Hui Y, Jin Y, Zhang J, Yang L, Sun X, Li X, Dong Q, Zhang J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Apr 14;:e20000

Neutrophil extracellular traps regulate LDHA expression to promote colorectal cancer liver metastasis.

Li N, Yang S, Hu C, Zheng J, Yang H, Jiang T, Li X, Han Q, Zhang X, Yang Y, Khan S, Sun B
Journal of translational medicine 2025 Nov 3;23(1):1208

Clinical Significance of Coiled-Coil Domain-Containing Protein 25 Expression in Esophageal Squamous Cell Carcinoma.

Suzuki T, Tsujimoto H, Watanabe T, Ishibashi Y, Fujishima S, Itazaki Y, Kariya R, Uehata N, Shinada H, Mochizuki S, Kishi Y, Sato K, Ueno H
Annals of surgical oncology 2025 May;32(5):3839-3850

The NET-DNA-CCDC25 inhibitor di-Pal-MTO suppresses tumor progression and promotes the innate immune response.

Wang S, Liang X, Li H, Zou J, Xu L, Zhang Y, Lin J, Zeng J, Zhong X, Liu X, Liu Z, Zheng Y, Nie M, Yang L
Cellular & molecular immunology 2025 Jun;22(6):628-644

Neutrophil extracellular trap gene expression signatures identify prognostic and targetable signaling axes for inhibiting pancreatic tumour metastasis.

McDonald PC, Topham JT, Awrey S, Tavakoli H, Carroll R, Brown WS, Gerbec ZJ, Kalloger SE, Karasinska JM, Tang P, Goodwin R, Jones SJM, Laskin J, Marra MA, Morin GB, Renouf DJ, Schaeffer DF, Dedhar S
Communications biology 2025 Jul 4;8(1):1006

Cardiomyocyte-localized CCDC25 senses NET DNA to promote doxorubicin cardiotoxicity by activating autophagic flux.

Nie M, Lei D, Liu Z, Zeng K, Liang X, Huang P, Wang Y, Sun P, Yang H, Liu P, Nie R, Huang S, Song E, Jiang W, Li Z, Yang Y, Xia Y, Yang L
Nature cancer 2025 Aug;6(8):1400-1418

Allergens abrogate antiinflammatory DNA effects and unmask macrophage-driven neutrophilic asthma via ILC2/STING/TNF-α signaling.

Sripada A, Verma D, Varma R, Sirohi K, Kwiat C, Pathria M, Verma M, Sahu A, Guntur V, Manka L, Vestal B, Moore C, Martin RJ, Gorska MM, Cambier J, Getahun A, Alam R
The Journal of clinical investigation 2025 Aug 15;135(16)

Eosinophil extracellular traps drive T follicular helper cell differentiation via VIRMA-dependent MAF stabilization in bullous pemphigoid.

Shen S, Fang H, Li X, Zhou Y, Tang X, Miao H, Li L, Chen J, Xue K, Zhang C, Chu M, Pang B, Bai Y, Qiao H, Dang E, Shao S, Wang G
The Journal of allergy and clinical immunology 2025 Apr;155(4):1357-1370

Choline Phosphate-Grafted Nanozymes as Universal Extracellular Vesicle Probes for Bladder Cancer Detection.

Li Q, Zhan S, Yang X, Zhang Z, Sun N, Wang X, Kang J, Du R, Hong X, Yue M, Li X, Tang Y, Liu G, Liu Y, Liu D
ACS nano 2024 Jun 25;18(25):16113-16125

Role of CD61(+) low-density neutrophils in promoting hepatocellular carcinoma metastasis through CCDC25 upregulation.

Guan X, Lu Y, Wang C, Zhan P, Chen Z
International immunopharmacology 2024 Jun 15;134:112272

Coiled-coil domain containing 25 (CCDC25) regulates cell proliferation, migration, and invasion in clear cell renal cell carcinoma by targeting the ILK-NF-κB signaling pathway.

Qian Z, Zhao H, Zhang Y, Wang Z, Zeng F, Zhu Y, Yang Y, Li J, Ma T, Huang C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jan 31;38(2):e23414

Neutrophil extracellular traps with low concentrations induce proliferation and migration of human fibroblasts via activating CCDC25/ILK/PI3K/AKT pathway.

Chu Z, Zheng Y, Shen Q, Yang K, Wang H, Ma K, Hao Y, Chen J, Li H, Fu X, Zhang C
Biochemical and biophysical research communications 2024 Dec 17;738:150954

Cell membrane derived liposomes loaded with DNase I target neutrophil extracellular traps which inhibits colorectal cancer liver metastases.

Wang Z, Chen C, Shi C, Zhao X, Gao L, Guo F, Han M, Yang Z, Zhang J, Tang C, Zhang C, Liu Y, Sun P, Jiang X
Journal of controlled release : official journal of the Controlled Release Society 2023 May;357:620-629

Neutrophil extracellular traps promote angiogenesis in gastric cancer.

Yang S, Sun B, Li J, Li N, Zhang A, Zhang X, Yang H, Zou X
Cell communication and signaling : CCS 2023 Jul 21;21(1):176

Ccdc25 regulates neurogenesis during the brain development.

Wang C, Qin J, Jiao J, Ji F
Developmental neurobiology 2023 Apr-May;83(3-4):91-103

Eosinophil extracellular traps drive asthma progression through neuro-immune signals.

Lu Y, Huang Y, Li J, Huang J, Zhang L, Feng J, Li J, Xia Q, Zhao Q, Huang L, Jiang S, Su S
Nature cell biology 2021 Oct;23(10):1060-1072

DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.

Yang L, Liu Q, Zhang X, Liu X, Zhou B, Chen J, Huang D, Li J, Li H, Chen F, Liu J, Xing Y, Chen X, Su S, Song E
Nature 2020 Jul;583(7814):133-138

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 21209-1-AP(CCDC25 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using CCDC25 antibody 21209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 21209-1-AP(CCDC25 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 21209-1-AP(CCDC25 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue