21209-1-AP

antibody from Proteintech Group

Targeting: CCDC25 FLJ10853

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21209-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#21209-1-AP, RRID:AB_10732690

Product name

CCDC25 antibody

Antibody type

Polyclonal

Description

KD/KO validated CCDC25 antibody (Cat. #21209-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Role of CD61(+) low-density neutrophils in promoting hepatocellular carcinoma metastasis through CCDC25 upregulation.

Guan X, Lu Y, Wang C, Zhan P, Chen Z
International immunopharmacology 2024 Jun 15;134:112272

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Coiled-coil domain containing 25 (CCDC25) regulates cell proliferation, migration, and invasion in clear cell renal cell carcinoma by targeting the ILK-NF-κB signaling pathway.

Qian Z, Zhao H, Zhang Y, Wang Z, Zeng F, Zhu Y, Yang Y, Li J, Ma T, Huang C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jan 31;38(2):e23414

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Cell membrane derived liposomes loaded with DNase I target neutrophil extracellular traps which inhibits colorectal cancer liver metastases.

Wang Z, Chen C, Shi C, Zhao X, Gao L, Guo F, Han M, Yang Z, Zhang J, Tang C, Zhang C, Liu Y, Sun P, Jiang X
Journal of controlled release : official journal of the Controlled Release Society 2023 May;357:620-629

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Neutrophil extracellular traps promote angiogenesis in gastric cancer.

Yang S, Sun B, Li J, Li N, Zhang A, Zhang X, Yang H, Zou X
Cell communication and signaling : CCS 2023 Jul 21;21(1):176

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Ccdc25 regulates neurogenesis during the brain development.

Wang C, Qin J, Jiao J, Ji F
Developmental neurobiology 2023 Apr-May;83(3-4):91-103

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Eosinophil extracellular traps drive asthma progression through neuro-immune signals.

Lu Y, Huang Y, Li J, Huang J, Zhang L, Feng J, Li J, Xia Q, Zhao Q, Huang L, Jiang S, Su S
Nature cell biology 2021 Oct;23(10):1060-1072

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DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.

Yang L, Liu Q, Zhang X, Liu X, Zhou B, Chen J, Huang D, Li J, Li H, Chen F, Liu J, Xing Y, Chen X, Su S, Song E
Nature 2020 Jul;583(7814):133-138

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 21209-1-AP(CCDC25 antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using CCDC25 antibody 21209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 21209-1-AP(CCDC25 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 21209-1-AP(CCDC25 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue