MA5-13871

antibody from Invitrogen Antibodies

Targeting: MVP LRP, VAULT1

Western blot (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Gel shift (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-13871

Provider

Invitrogen Antibodies

Product name

MVP Monoclonal Antibody (1032)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-13871 targets Major Vault Protein in FACS, GS, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples. The MA5-13871 immunogen is proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

1032

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

Proteome Profiling of RNF213 Depleted Cells Reveals Nitric Oxide Regulator DDAH1 Antilisterial Activity.

Martina L, Asselman C, Thery F, Boucher K, Delhaye L, Maia TM, Dermaut B, Eyckerman S, Impens F
Frontiers in cellular and infection microbiology 2021;11:735416

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MVP expression in the prediction of clinical outcome of locally advanced oral squamous cell carcinoma patients treated with radiotherapy.

Henríquez-Hernández LA, Moreno M, Rey A, Lloret M, Lara PC
Radiation oncology (London, England) 2012 Aug 29;7:147

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Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line.

Guan J, Chen XP, Zhu H, Luo SF, Cao B, Ding L
World journal of gastroenterology 2004 Dec 1;10(23):3522-7

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Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line.

Guan J, Chen XP, Zhu H, Luo SF, Cao B, Ding L
World journal of gastroenterology 2004 Dec 1;10(23):3522-7

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human breast carcinoma stained with LRP/MVP antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 RNF213 knockdown downregulates DDAH1 and CYR61 at the transcript level. HeLa cells were treated with IFN-beta in combination with knockdown of RNF213 as described in Figure 1 . (A) Immunoblotting on the actual samples used for proteome analysis confirmed efficient knockdown of RNF213 and interferon-induced upregulation of free and conjugated ISG15. Immunoblots against DDAH1, CYR61 and MVP validated downregulation of DDAH1 and CYR61, and upregulation of MVP upon knockdown of RNF213. Tubulin was used as loading control. Densitometry and statistical analysis on two more biological repeats confirmed significant up- or downregulation of the immunoblotting bands ( Supplementary Figure 3 ). (B) Real time qPCR upon knockdown of RNF213 showed significant upregulation of MVP in the presence of IFN-beta and significant downregulation of CYR61 and DDAH1 transcripts in the presence and absence of IFN-beta. Expression was normalized to housekeeping genes and shown relative to control (siScramble) populations set to 1. Data represents 3 biological replicates with 4 technical replicates per condition. (AVG +- SEM, two-tailed unpaired t-test (n = 3), *p < 0.05 and ***p < 0.001).