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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21729 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GAS2L1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, A375, mouse brain. Predicted reactivity: Mouse (86%), Rat (86%), Pig (91%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GAS2L1 Polyclonal Antibody (Product # PA5-21729). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. GAS2L1 Polyclonal Antibody (Product # PA5-21729) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GAS2L1 Polyclonal Antibody detects GAS2L1 protein by Western blot analysis. A. 30 µg A431 whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract. C. 30 µg A375 whole cell lysate/extract.10 % SDS-PAGE. GAS2L1 Polyclonal Antibody (Product # PA5-21729) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GAS2L1 in methanol-fixed A431 cells using a GAS2L1 polyclonal antibody (Product # PA5-21729) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using GAS2L1 (Product # PA5-21729) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using GAS2L1 (Product # PA5-21729) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
