LS-C185691

antibody from LSBio

Targeting: NR0B1 AHC, AHCH, DAX1, DSS

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C185691

Provider

LSBio

Product name

NR0B1 / DAX1 Antibody (aa96-406) LS-C185691

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: HeLa S3 . 7.5% SDS PAGE. NR0B1 / DAX1 antibody diluted at 1:500

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence of paraformaldehyde-fixed HeLa using DAX-1 antibody at 1:200 dilution.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence of paraformaldehyde-fixed HeLa using DAX-1 antibody at 1:200 dilution.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

NRB1 antibody immunoprecipitates NRB1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of NRB1 protein by 2 ug of NRB1 antibody 7.5% SDS-PAGE The immunoprecipitated NRB1 protein was detected by NRB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

NRB1 antibody immunoprecipitates NRB1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of NRB1 protein by 2 ug of NRB1 antibody 7.5% SDS-PAGE The immunoprecipitated NRB1 protein was detected by NRB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

NRB1 antibody immunoprecipitates NRB1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of NRB1 protein by 2 ug of NRB1 antibody 7.5% SDS-PAGE The immunoprecipitated NRB1 protein was detected by NRB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Cal27 xenograft using NR0B1 antibody at 1:100 dilution.