MA5-34635

antibody from Invitrogen Antibodies

Targeting: NR0B1 AHC, AHCH, DAX1, DSS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34635

Provider

Invitrogen Antibodies

Product name

NR0B1 Recombinant Rabbit Monoclonal Antibody (JU33-05)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: A549, Hela, SH-SY-5Y.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JU33-05

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NR0B1 in A549 cell lysate. Samples were incubated with NR0B1 monoclonal antibody (Product # MA5-34635), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NR0B1 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NR0B1 monoclonal antibody (Product # MA5-34635), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NR0B1 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NR0B1 monoclonal antibody (Product # MA5-34635), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NR0B1 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NR0B1 monoclonal antibody (Product # MA5-34635), followed by DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of NR0B1 in A549 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with NR0B1 monoclonal antibody (Product # MA5-34635) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.