LS-C744677

antibody from LSBio

Targeting: GK GK1, GKD

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C744677

Provider

LSBio

Product name

GK / Glycerol Kinase Antibody (Liquid) LS-C744677

Antibody type

Polyclonal

Description

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Host

Goat

Isotype

IgG

Storage

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Goat anti Glycerol Kinase antibody was used to detect purified Glycerol Kinase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Both the antiserum and IgG fractions of anti-Glycerol Kinase (Cellulomonas) are shown to detect the 128,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:4,000 dilution of the primary antibody is used followed by detection using HRP Rabbit-a-Goat IgG [H&L] (605-4302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.