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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- R1089 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1089, RRID:AB_1001920
- Product name
- anti Glycerol kinase
- Antibody type
- Polyclonal
- Antigen
- Glycerol Kinase [Cellulomonas species]
- Host
- Goat
- Vial size
- 2 ml
- Concentration
- 90 mg/ml (by refractometry)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Both the antiserum and IgG fractions of anti-Glycerol Kinase (Cellulomonas) are shown to detect the 128,000 dalton enzyme in cellular extracts. Approximately 10 μg of total protein is loaded per lane. A 1:4,000 dilution of the primary antibody is used followed by detection using HRP Rabbit-a-Goat IgG [H&L] diluted 1:4,000 and colordevelopment using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
