PA5-55721

antibody from Invitrogen Antibodies

Targeting: NUP153 HNUP153

Antibody data

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Validation data

Product number: PA5-55721 - Provider product page
Provider: Invitrogen Antibodies
Product name: NUP153 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Immunogen sequence: SSRASDKDIT VSKNTSLPPL WSPEAERSHS LSQHTATSSK KPAFNLSAFG TLSPSLGNSS ILKTSQLGDS PFYPGKTTYG GAAAAVRQSK LRNTP Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 93%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 0.1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

NUP153 protein structure - PA5-55721 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of NUP153 in human cell line A-431 shows positivity in nuclear membrane. Samples were probed using a NUP153 Polyclonal Antibody (Product # PA5-55721).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of NUP153 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NUP153 Rabbit Polyclonal Antibody (Product # PA5-55721) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear and Nuclear membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of NUP153 was achieved by transfecting HeLa cells with NUP153 specific siRNA (Silencer® select Product # s19374). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with NUP153 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with NUP153 Rabbit Polyclonal Antibody (Product # PA5-55721, 5 µg/mL), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Reduction of specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to NUP153 (green). The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of NUP153 was achieved by transfecting HeLa cells with NUP153 specific siRNA (Silencer® select Product # s19374). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with NUP153 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with NUP153 Rabbit Polyclonal Antibody (Product # PA5-55721, 5 µg/mL), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Reduction of specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to NUP153 (green). The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of NUP153 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NUP153 Rabbit Polyclonal Antibody (Product # PA5-55721) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear and Nuclear membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorecent analysis of NUP153 in human cell line A-431 using NUP153 Polyclonal Antibody (Product # PA5-55721). Staining shows localization to nuclear membrane.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of NUP153 in human pancreas using NUP153 Polyclonal Antibody (Product # PA5-55721) shows very weak positivity in nuclear membrane in exocrine glandular cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of NUP153 in human cerebral cortex using NUP153 Polyclonal Antibody (Product # PA5-55721) shows moderate to strong positivity in nuclear membrane in neurons.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of NUP153 in human endometrium using NUP153 Polyclonal Antibody (Product # PA5-55721) shows weak to moderate positivity in nuclear membrane in glandular cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of NUP153 in human testis using NUP153 Polyclonal Antibody (Product # PA5-55721) shows moderate positivity in nuclear membrane in cells in seminiferous ducts.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of NUP153 in human pancreas using NUP153 Polyclonal Antibody (Product # PA5-55721) shows very weak positivity in nuclear membrane in exocrine glandular cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of NUP153 in human cerebral cortex using NUP153 Polyclonal Antibody (Product # PA5-55721) shows moderate to strong positivity in nuclear membrane in neurons.