10538-1-AP

antibody from Proteintech Group

Targeting: CAPN1 CANP, CANPL1, muCANP, muCL

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10538-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10538-1-AP, RRID:AB_10858627

Product name

Calpain 1 antibody

Antibody type

Polyclonal

Description

KD/KO validated Calpain 1 antibody (Cat. #10538-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Astragaloside IV prevents calpain-1-mediated cardiac hypertrophy and fibrosis induced by diabetes.

Zhang Q, Zhao N, Wei S, Lu M, Chen K
Frontiers in cardiovascular medicine 2026;13:1670472

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RIPK1-driven calcium overload and lysosomal-mitochondrial dysfunction induce testicular necroptosis following DEHP exposure.

Ran M, Yi R, Zhang H, Sun X, Qiu T, Li J, Wang N, Yao X, Zhang C, Deng H, Wang S, Yang G
Ecotoxicology and environmental safety 2026 May;316:120163

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Dietary supplementation with pterostilbene improves the water-holding capacity of pork in finishing pigs.

Huang T, Chen X, Yu B, Zheng P, Chen D, Huang Z
Journal of the science of food and agriculture 2026 Mar 30;106(5):3178-3190

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Calpain-1 Potentiates Periodontal Regeneration via PHLPP1-ERK-Driven Osteogenesis in Periodontal Ligament Stem Cells.

Li Y, Yang B, Mei Z, Deng P, Fu T, Li J
International dental journal 2026 Feb;76(1):109309

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Taltirelin treatment alleviates PTSD-like symptoms and restores neural oscillations in male mice receiving single prolonged stress.

Ding K, Zhang Z, Niu J, Zhu M, Zhang J, Chen L, Yang S, Wang J, Zhu G
Neuropharmacology 2026 Feb 15;284:110791

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Loss-of-function variants in the CAPN1 activator CD99L2 cause X-linked spastic ataxia.

Menden B, Incebacak Eltemur RD, Demidov G, Sturm M, Park J, Huridou C, Fath F, Nümann A, Baumann A, Diets IJ, Dufke C, Regensburger M, Rönnefarth M, Wilke V, van Os N, Vielhaber S, Rattay TW, Kohl Z, Peralta S, Pereira Sena P, Kellner M, Weissert N, Traschütz A, Zeltner L, Boelmans K, Deininger N, Schütz L, Gross C, Hinojosa Amaya AB, Raupach K, Hengel H, Harmuth F, Admard J, Bader I, Baumann S, Bender F, Bevot A, Bischoff A, Boschann F, Buchert R, Buchzik D, Casadei N, Catarino CB, Cordts I, Cremer K, Doebler-Neumann M, Ehmke N, Elbracht M, Falb RJ, Feindt T, Fleszar Z, Gerstner L, Gläser D, Grasshoff U, Grosch S, Grundmann K, Gutschalk A, Haaga M, Hayer S, Hehr U, Hellenbroich Y, Henn W, Herr B, Herzog R, Horber V, Deppe J, Kaiser N, Kehrer C, Kehrer M, Kern J, Keßler C, Khuller K, Klinkhammer H, Kotzaeridou U, Krawitz P, Kreiss M, Küpper H, Kuster A, Laugwitz L, Lesemann A, Lichey N, Linden T, Macek B, Magg J, Mangold E, Manka E, Marquardt I, Mehnert K, Mengel D, Morlot S, Oehl-Jaschkowitz B, Pauly MG, Philipp M, Radelfahr F, Rautenberg M, Riess A, Saft C, Schlotter-Weigel B, Schmidt A, Schwaibold EMC, Spahlinger V, Spranger S, Steiner KM, Stendel C, Thieme A, Tzschach A, Velic A, Wiethoff S, Wilke C, Züchner S, Zittel S, Solve-RD consortium, Husain RA, Deschauer M, Distelmaier F, Dufke A, Graessner H, Hemmer B, Jacobi H, Klockgether T, Klopstock T, Kobeleva X, Korenke GC, Kuechler A, Kuhlenbäumer G, Kurth I, Nguyen HP, Wunderlich G, Zeuner KE, Klebe S, Auer-Grumbach M, Butryn M, Winkler J, Timmann D, Synofzik M, van de Warrenburg B, Schüle R, Schöls L, Ossowski S, Riess O, Weber JJ, Haack TB
Nature communications 2026 Feb 14;17(1):1698

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Effects of dietary grape seed proanthocyanidin extract supplementation on muscle water-holding capacity of finishing pigs.

Yu H, Chen X, He J, Zheng P, Luo Y, Yu B, Chen D, Huang Z
Meat science 2025 Sep;227:109865

---

MSC-EVs alleviate sepsis-induced acute lung injury through inhibiting NET-triggered damage to adherens junctions of endothelial cells.

Shao Y, Wu D, Miao C, Zhang H
Molecular immunology 2025 Sep;185:6-17

---

TRIM45 restricts influenza virus infection through modulating the chaperone-mediated autophagic degradation of viral PB2 protein.

Wang Y, Jiang L, Li Q, Li M, Shi W, Wang B, Wang G, Deng G, Shi J, Tian G, Zeng X, Chen H, Li C
PLoS pathogens 2025 Oct;21(10):e1013630

---

Cinobufagin overcomes bortezomib resistance in multiple myeloma strains by targeting SEC62/TRPM4-mediated NECSO.

Yin Z, Li G, Zhong Q, Zhang X, Ou R, Shen H, Huang J, Mahati S, Liu Z, Zhu Y, Zhang Q, Liu S
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Nov;147:157171

---

Single-atom Ca nanozyme induces glioma death through Ca(2+)-overload-enhanced catalytic tumor nanotherapy, ferroptosis and synergistic remodeling of the immune microenvironment.

Jin Z, Li X, Hou X, Hu J, Zhuo Y, Shi J, Xu T, Zhao Q, Jing Z
Journal of nanobiotechnology 2025 Nov 13;23(1):716

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Influenza A Virus Induces Autophagosome by Inhibiting LTCC/Calpain 2/LC3A Signaling to Promote Viral Replication.

Tian L, Liu X, Zheng F, Ren Z, Li T, Zhang Z, Zhu L, Chen W, Li K, Sheng J
Journal of medical virology 2025 May;97(5):e70393

---

Calpain1 inhibition enhances autophagy-lysosomal pathway and ameliorates tubulointerstitial fibrosis in Nephronophthisis.

Li D, Zhang J, Su X, Yang Y, Lai J, Wei X, Chen H, Liu Y, Wang H, Sun L
Molecular medicine (Cambridge, Mass.) 2025 May 3;31(1):166

---

Ethanol extract of Moschus attenuates glutamate-induced cytotoxicity in HT22 cells by regulating the Nrf2 and MAPK pathways.

Chu Z, Chen Y, Xie D, Song C, Yang L, Qin T, Zhai Z, Cao Z, Xu Y, Sun T
Journal of ethnopharmacology 2025 May 28;348:119879

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Calpain-2-Mediated Endothelial Focal Adhesion Disruption in Thoracic Aortic Dissection.

Teng X, Wang Y, Huang H, Ding Y, Wang J, Liu M, Song K, Shao L, Yu Y, Yang Z, Shen Z
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jul;12(25):e2501112

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Calpains orchestrate secretion of annexin-containing microvesicles during membrane repair.

Williams JK, Ngo JM, Murugupandiyan A, Croall DE, Hartzell HC, Schekman R
The Journal of cell biology 2025 Jul 7;224(7)

---

Astragaloside IV improves cognitive impairment in diabetes by inhibiting calpain-1/NLRP3 mediated microglial activation.

Wang X, Jiang H, Wang H, Zhao C, Ba X
International immunopharmacology 2025 Dec 10;167:115682

---

Calpain inhibition as a novel therapeutic strategy for aortic dissection with acute lower extremity ischemia.

Tan Q, Wang X, Xu W, Song K, Xiong Y, Jiang Z, Li J, Yu Y, Ye W, Shen Z, Teng X
Molecular medicine (Cambridge, Mass.) 2025 Apr 21;31(1):144

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DCC, a potential target for controlling fear memory extinction and hippocampal LTP in male mice receiving single prolonged stress.

Yang S, Hu J, Chen Y, Zhang Z, Wang J, Zhu G
Neurobiology of stress 2024 Sep;32:100666

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The RhoB p.S73F mutation leads to cerebral palsy through dysregulation of lipid homeostasis.

Wu X, Liu R, Zhang Z, Yang J, Liu X, Jiang L, Fang M, Wang S, Lai L, Song Y, Li Z
EMBO molecular medicine 2024 Sep;16(9):2002-2023

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Calpains Orchestrate Secretion of Annexin-containing Microvesicles during Membrane Repair.

Williams JK, Ngo JM, Murugupandiyan A, Croall DE, Hartzell HC, Schekman R
bioRxiv : the preprint server for biology 2024 Sep 6;

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TRPC6-Calpain-1 Axis Promotes Tubulointerstitial Inflammation by Inhibiting Mitophagy in Diabetic Kidney Disease.

Liu CC, Ji JL, Wang Z, Zhang XJ, Ding L, Zhang Y, Zhou Y, Zhang DJ, Tang ZL, Cao JY, Zhang AQ, Liu BC, Li ZL, Ma RX
Kidney international reports 2024 Nov;9(11):3301-3317

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Otud6b induces pulmonary arterial hypertension by mediating the Calpain-1/HIF-1α signaling pathway.

Liu Y, Tang B, Wang H, Lu M
Cellular and molecular life sciences : CMLS 2024 Jun 15;81(1):258

---

Ginsenoside Rg1 alleviates vascular remodeling in hypoxia-induced pulmonary hypertension mice through the calpain-1/STAT3 signaling pathway.

Ran C, Lu M, Zhao F, Hao Y, Guo X, Li Y, Su Y, Wang H
Journal of ginseng research 2024 Jul;48(4):405-416

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Calpain-1 Up-Regulation Promotes Bleomycin-Induced Pulmonary Fibrosis by Activating Ferroptosis.

Wei S, Liu Y, Ran C, Li Y, Tang B, Lu M, Wang H
The American journal of pathology 2024 Dec;194(12):2272-2289

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Effects of chronic heat stress on Ca(2+) homeostasis, apoptosis, and protein carbonylation profiles in the breast muscle of broilers.

Liu Y, Liu Z, Xing T, Li J, Zhang L, Zhao L, Gao F
Poultry science 2024 Dec;103(12):104342

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The Role of Endophilin A1 in Lipopolysaccharide-Induced Parkinson's Disease Model Mice.

Han J, Liu M, Ling Y, Ren Y, Qiu Y, Liu Y, Yin Y
Journal of Parkinson's disease 2023;13(5):743-756

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Ginsenoside Rg1 attenuates diabetic vascular endothelial dysfunction by inhibiting the calpain-1/ROS/PKC-β axis.

Lu M, Zhao F, Ran C, Xu Y, Zhang J, Wang H
Life sciences 2023 Sep 15;329:121972

---

Downregulation of NHE1 expression attenuates apoptosis of primary hippocampal neurons of an epilepsy model through the calpain-1 pathway.

Wu X, Xie D, Zheng Q, Peng S, Liu Y, Ma P, Ye L, Mo X, Feng Z
Neuroscience letters 2023 Oct 15;815:137494

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The role of EndophilinA1 in chronic unpredicted mild stress-induced depression model mice.

Liu M, Ling Y, Zhang Y, Liu L, Qiu Y, Liu Y, Yin Y
International immunopharmacology 2023 Nov;124(Pt B):111023

---

Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.

Liu X, Jiang L, Zhang W, Zhang J, Luan X, Zhan Y, Wang T, Da J, Liu L, Zhang S, Guo Y, Zhang K, Wang Z, Miao N, Xie X, Liu P, Li Y, Jin H, Zhang B
Journal of translational medicine 2023 Jun 27;21(1):417

---

Aberrant mechanical loading induces annulus fibrosus cells apoptosis in intervertebral disc degeneration via mechanosensitive ion channel Piezo1.

Liu C, Gao X, Lou J, Li H, Chen Y, Chen M, Zhang Y, Hu Z, Chang X, Luo M, Zhai Y, Li C
Arthritis research & therapy 2023 Jul 7;25(1):117

---

Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway.

Zhao F, Lu M, Wang H
Journal of ginseng research 2023 Jan;47(1):144-154

---

Fear extinction induced by activation of PKA ameliorates anxiety-like behavior in PTSD mice.

Gao F, Wang J, Yang S, Ji M, Zhu G
Neuropharmacology 2023 Jan 1;222:109306

---

Protective effect of Astragaloside IV on chronic intermittent hypoxia-induced vascular endothelial dysfunction through the calpain-1/SIRT1/AMPK signaling pathway.

Zhao F, Meng Y, Wang Y, Fan S, Liu Y, Zhang X, Ran C, Wang H, Lu M
Frontiers in pharmacology 2022;13:920977

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Blood plasma derived extracellular vesicles (BEVs): particle purification liquid chromatography (PPLC) and proteomic analysis reveals BEVs as a potential minimally invasive tool for predicting response to breast cancer treatment.

Alvarez FA, Kaddour H, Lyu Y, Preece C, Cohen J, Baer L, Stopeck AT, Thompson P, Okeoma CM
Breast cancer research and treatment 2022 Nov;196(2):423-437

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Calpain-1 mediates vascular remodelling and fibrosis via HIF-1α in hypoxia-induced pulmonary hypertension.

Deng H, Tian X, Sun H, Liu H, Lu M, Wang H
Journal of cellular and molecular medicine 2022 May;26(10):2819-2830

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TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.

Tian W, Liu SY, Zhang M, Meng JR, Tang N, Feng YD, Sun Y, Gao YY, Zhou L, Cao W, Li XQ
Pharmacological research 2022 Jul;181:106262

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Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.

Wang J, Gao F, Cui S, Yang S, Gao F, Wang X, Zhu G
Pharmacological research 2022 Feb;176:106079

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Acid-Sensing Ion Channel 1/Calpain1 Activation Impedes Macrophage ATP-Binding Cassette Protein A1-Mediated Cholesterol Efflux Induced by Extracellular Acidification.

Wang YM, Tan MY, Zhang RJ, Qiu MY, Fu YS, Xie XJ, Gu HF
Frontiers in physiology 2021;12:777386

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miR‑140‑5p alleviates mouse liver ischemia/reperfusion injury by targeting CAPN1.

Yu Q, Chen S, Tang H, Yang H, Zhang J, Shi X, Li J, Guo W, Zhang S
Molecular medicine reports 2021 Sep;24(3)

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Astrocytic Kir4.1 regulates NMDAR/calpain signaling axis in lipopolysaccharide-induced depression-like behaviors in mice.

Song Z, Bian Z, Zhang Z, Wang X, Zhu A, Zhu G
Toxicology and applied pharmacology 2021 Oct 15;429:115711

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The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.

Kotani T, Takegaki J, Tamura Y, Kouzaki K, Nakazato K, Ishii N
Physiological reports 2021 May;9(9):e14842

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Inactivation of cysteine 674 in the sarcoplasmic/endoplasmic reticulum calcium ATPase 2 causes retinopathy in the mouse.

Liu G, Wu F, Wu H, Wang Y, Jiang X, Hu P, Tong X
Experimental eye research 2021 Jun;207:108559

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NCX3 alleviates ethanol-induced apoptosis of SK-N-SH cells via the elimination of intracellular calcium ions.

Xia Z, Wang C, Wang X, Yu H, Yao H, Shen H, Lan X, Wu X, Zhang G
Toxicology in vitro : an international journal published in association with BIBRA 2021 Apr;72:105104

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Over-activation of NMDA receptors promotes ABCA1 degradation and foam cell formation.

Cheng H, Cheng Q, Bao X, Luo Y, Zhou Y, Li Y, Hua Q, Liu W, Tang S, Feng D, Luo Z
Biochimica et biophysica acta. Molecular and cell biology of lipids 2020 Oct;1865(10):158778

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Elastic titin properties and protein quality control in the aging heart.

Salcan S, Bongardt S, Monteiro Barbosa D, Efimov IR, Rassaf T, Krüger M, Kötter S
Biochimica et biophysica acta. Molecular cell research 2020 Mar;1867(3):118532

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CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.

Chen Y, Tang J, Lu T, Liu F
Thoracic cancer 2020 Jul;11(7):1848-1860

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Astragaloside IV protects against hyperglycemia-induced vascular endothelial dysfunction by inhibiting oxidative stress and Calpain-1 activation.

Nie Q, Zhu L, Zhang L, Leng B, Wang H
Life sciences 2019 Sep 1;232:116662

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Suppressing the Na(+)/H(+) exchanger 1: a new sight to treat depression.

Deng X, Ji Z, Xu B, Guo L, Xu L, Qin T, Feng L, Ma Z, Fu Q, Qu R, Quo Q, Ma S
Cell death & disease 2019 May 8;10(5):370

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Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Wang S, Huang Y, Yan Y, Zhou H, Wang M, Liao L, Wang Z, Chen D, Ji D, Xia X, Liu F, Huang J, Xiong K
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2019 Jan;221:57-67

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Triptolide protects against TGF-β1-induced pulmonary fibrosis by regulating FAK/calpain signaling.

Zhang P, Liu J, Zong R
Experimental and therapeutic medicine 2019 Dec;18(6):4781-4789

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Expression of Cell Cycle-related Proteins p16, p27 and Ki-67 Proliferating Marker in Laryngeal Squamous Cell Carcinomas and in Laryngeal Papillomas.

Ciesielska U, Zatonski T, Nowinska K, Ratajczak-Wielgomas K, Grzegrzolka J, Piotrowska A, Olbromski M, Pula B, Podhorska-Okolow M, Dziegiel P
Anticancer research 2017 May;37(5):2407-2415

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Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.

Zhang J, Lu S, Zhou Y, Meng K, Chen Z, Cui Y, Shi Y, Wang T, He QY
Proteomics 2017 Jul;17(13-14)

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Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.

Kmiecik AM, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski M, Podhorska-Okolow M, Dziegiel P
PloS one 2015;10(5):e0124865

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Calpain Cleaves Most Components in the Multiple Aminoacyl-tRNA Synthetase Complex and Affects Their Functions.

Lei HY, Zhou XL, Ruan ZR, Sun WC, Eriani G, Wang ED
The Journal of biological chemistry 2015 Oct 23;290(43):26314-27

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP(CAPN1 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using CAPN1 antibody 10538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 10538-1-AP(CAPN1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 10538-1-AP(CAPN1 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue