Antibody data
- Antibody Data
- Antigen structure
- References [31]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 10538-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10538-1-AP, RRID:AB_10858627
- Product name
- Calpain 1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Calpain 1 antibody (Cat. #10538-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Ginsenoside Rg1 alleviates vascular remodeling in hypoxia-induced pulmonary hypertension mice through the calpain-1/STAT3 signaling pathway.
The Role of Endophilin A1 in Lipopolysaccharide-Induced Parkinson's Disease Model Mice.
Ginsenoside Rg1 attenuates diabetic vascular endothelial dysfunction by inhibiting the calpain-1/ROS/PKC-β axis.
Downregulation of NHE1 expression attenuates apoptosis of primary hippocampal neurons of an epilepsy model through the calpain-1 pathway.
The role of EndophilinA1 in chronic unpredicted mild stress-induced depression model mice.
Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.
Aberrant mechanical loading induces annulus fibrosus cells apoptosis in intervertebral disc degeneration via mechanosensitive ion channel Piezo1.
Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway.
Fear extinction induced by activation of PKA ameliorates anxiety-like behavior in PTSD mice.
Protective effect of Astragaloside IV on chronic intermittent hypoxia-induced vascular endothelial dysfunction through the calpain-1/SIRT1/AMPK signaling pathway.
Blood plasma derived extracellular vesicles (BEVs): particle purification liquid chromatography (PPLC) and proteomic analysis reveals BEVs as a potential minimally invasive tool for predicting response to breast cancer treatment.
Calpain-1 mediates vascular remodelling and fibrosis via HIF-1α in hypoxia-induced pulmonary hypertension.
TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.
Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.
Acid-Sensing Ion Channel 1/Calpain1 Activation Impedes Macrophage ATP-Binding Cassette Protein A1-Mediated Cholesterol Efflux Induced by Extracellular Acidification.
miR‑140‑5p alleviates mouse liver ischemia/reperfusion injury by targeting CAPN1.
Astrocytic Kir4.1 regulates NMDAR/calpain signaling axis in lipopolysaccharide-induced depression-like behaviors in mice.
The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.
Inactivation of cysteine 674 in the sarcoplasmic/endoplasmic reticulum calcium ATPase 2 causes retinopathy in the mouse.
NCX3 alleviates ethanol-induced apoptosis of SK-N-SH cells via the elimination of intracellular calcium ions.
Over-activation of NMDA receptors promotes ABCA1 degradation and foam cell formation.
Elastic titin properties and protein quality control in the aging heart.
CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.
Astragaloside IV protects against hyperglycemia-induced vascular endothelial dysfunction by inhibiting oxidative stress and Calpain-1 activation.
Suppressing the Na(+)/H(+) exchanger 1: a new sight to treat depression.
Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.
Triptolide protects against TGF-β1-induced pulmonary fibrosis by regulating FAK/calpain signaling.
Expression of Cell Cycle-related Proteins p16, p27 and Ki-67 Proliferating Marker in Laryngeal Squamous Cell Carcinomas and in Laryngeal Papillomas.
Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.
Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.
Calpain Cleaves Most Components in the Multiple Aminoacyl-tRNA Synthetase Complex and Affects Their Functions.
Ran C, Lu M, Zhao F, Hao Y, Guo X, Li Y, Su Y, Wang H
Journal of ginseng research 2024 Jul;48(4):405-416
Journal of ginseng research 2024 Jul;48(4):405-416
The Role of Endophilin A1 in Lipopolysaccharide-Induced Parkinson's Disease Model Mice.
Han J, Liu M, Ling Y, Ren Y, Qiu Y, Liu Y, Yin Y
Journal of Parkinson's disease 2023;13(5):743-756
Journal of Parkinson's disease 2023;13(5):743-756
Ginsenoside Rg1 attenuates diabetic vascular endothelial dysfunction by inhibiting the calpain-1/ROS/PKC-β axis.
Lu M, Zhao F, Ran C, Xu Y, Zhang J, Wang H
Life sciences 2023 Sep 15;329:121972
Life sciences 2023 Sep 15;329:121972
Downregulation of NHE1 expression attenuates apoptosis of primary hippocampal neurons of an epilepsy model through the calpain-1 pathway.
Wu X, Xie D, Zheng Q, Peng S, Liu Y, Ma P, Ye L, Mo X, Feng Z
Neuroscience letters 2023 Oct 15;815:137494
Neuroscience letters 2023 Oct 15;815:137494
The role of EndophilinA1 in chronic unpredicted mild stress-induced depression model mice.
Liu M, Ling Y, Zhang Y, Liu L, Qiu Y, Liu Y, Yin Y
International immunopharmacology 2023 Nov;124(Pt B):111023
International immunopharmacology 2023 Nov;124(Pt B):111023
Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.
Liu X, Jiang L, Zhang W, Zhang J, Luan X, Zhan Y, Wang T, Da J, Liu L, Zhang S, Guo Y, Zhang K, Wang Z, Miao N, Xie X, Liu P, Li Y, Jin H, Zhang B
Journal of translational medicine 2023 Jun 27;21(1):417
Journal of translational medicine 2023 Jun 27;21(1):417
Aberrant mechanical loading induces annulus fibrosus cells apoptosis in intervertebral disc degeneration via mechanosensitive ion channel Piezo1.
Liu C, Gao X, Lou J, Li H, Chen Y, Chen M, Zhang Y, Hu Z, Chang X, Luo M, Zhai Y, Li C
Arthritis research & therapy 2023 Jul 7;25(1):117
Arthritis research & therapy 2023 Jul 7;25(1):117
Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway.
Zhao F, Lu M, Wang H
Journal of ginseng research 2023 Jan;47(1):144-154
Journal of ginseng research 2023 Jan;47(1):144-154
Fear extinction induced by activation of PKA ameliorates anxiety-like behavior in PTSD mice.
Gao F, Wang J, Yang S, Ji M, Zhu G
Neuropharmacology 2023 Jan 1;222:109306
Neuropharmacology 2023 Jan 1;222:109306
Protective effect of Astragaloside IV on chronic intermittent hypoxia-induced vascular endothelial dysfunction through the calpain-1/SIRT1/AMPK signaling pathway.
Zhao F, Meng Y, Wang Y, Fan S, Liu Y, Zhang X, Ran C, Wang H, Lu M
Frontiers in pharmacology 2022;13:920977
Frontiers in pharmacology 2022;13:920977
Blood plasma derived extracellular vesicles (BEVs): particle purification liquid chromatography (PPLC) and proteomic analysis reveals BEVs as a potential minimally invasive tool for predicting response to breast cancer treatment.
Alvarez FA, Kaddour H, Lyu Y, Preece C, Cohen J, Baer L, Stopeck AT, Thompson P, Okeoma CM
Breast cancer research and treatment 2022 Nov;196(2):423-437
Breast cancer research and treatment 2022 Nov;196(2):423-437
Calpain-1 mediates vascular remodelling and fibrosis via HIF-1α in hypoxia-induced pulmonary hypertension.
Deng H, Tian X, Sun H, Liu H, Lu M, Wang H
Journal of cellular and molecular medicine 2022 May;26(10):2819-2830
Journal of cellular and molecular medicine 2022 May;26(10):2819-2830
TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.
Tian W, Liu SY, Zhang M, Meng JR, Tang N, Feng YD, Sun Y, Gao YY, Zhou L, Cao W, Li XQ
Pharmacological research 2022 Jul;181:106262
Pharmacological research 2022 Jul;181:106262
Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.
Wang J, Gao F, Cui S, Yang S, Gao F, Wang X, Zhu G
Pharmacological research 2022 Feb;176:106079
Pharmacological research 2022 Feb;176:106079
Acid-Sensing Ion Channel 1/Calpain1 Activation Impedes Macrophage ATP-Binding Cassette Protein A1-Mediated Cholesterol Efflux Induced by Extracellular Acidification.
Wang YM, Tan MY, Zhang RJ, Qiu MY, Fu YS, Xie XJ, Gu HF
Frontiers in physiology 2021;12:777386
Frontiers in physiology 2021;12:777386
miR‑140‑5p alleviates mouse liver ischemia/reperfusion injury by targeting CAPN1.
Yu Q, Chen S, Tang H, Yang H, Zhang J, Shi X, Li J, Guo W, Zhang S
Molecular medicine reports 2021 Sep;24(3)
Molecular medicine reports 2021 Sep;24(3)
Astrocytic Kir4.1 regulates NMDAR/calpain signaling axis in lipopolysaccharide-induced depression-like behaviors in mice.
Song Z, Bian Z, Zhang Z, Wang X, Zhu A, Zhu G
Toxicology and applied pharmacology 2021 Oct 15;429:115711
Toxicology and applied pharmacology 2021 Oct 15;429:115711
The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.
Kotani T, Takegaki J, Tamura Y, Kouzaki K, Nakazato K, Ishii N
Physiological reports 2021 May;9(9):e14842
Physiological reports 2021 May;9(9):e14842
Inactivation of cysteine 674 in the sarcoplasmic/endoplasmic reticulum calcium ATPase 2 causes retinopathy in the mouse.
Liu G, Wu F, Wu H, Wang Y, Jiang X, Hu P, Tong X
Experimental eye research 2021 Jun;207:108559
Experimental eye research 2021 Jun;207:108559
NCX3 alleviates ethanol-induced apoptosis of SK-N-SH cells via the elimination of intracellular calcium ions.
Xia Z, Wang C, Wang X, Yu H, Yao H, Shen H, Lan X, Wu X, Zhang G
Toxicology in vitro : an international journal published in association with BIBRA 2021 Apr;72:105104
Toxicology in vitro : an international journal published in association with BIBRA 2021 Apr;72:105104
Over-activation of NMDA receptors promotes ABCA1 degradation and foam cell formation.
Cheng H, Cheng Q, Bao X, Luo Y, Zhou Y, Li Y, Hua Q, Liu W, Tang S, Feng D, Luo Z
Biochimica et biophysica acta. Molecular and cell biology of lipids 2020 Oct;1865(10):158778
Biochimica et biophysica acta. Molecular and cell biology of lipids 2020 Oct;1865(10):158778
Elastic titin properties and protein quality control in the aging heart.
Salcan S, Bongardt S, Monteiro Barbosa D, Efimov IR, Rassaf T, Krüger M, Kötter S
Biochimica et biophysica acta. Molecular cell research 2020 Mar;1867(3):118532
Biochimica et biophysica acta. Molecular cell research 2020 Mar;1867(3):118532
CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.
Chen Y, Tang J, Lu T, Liu F
Thoracic cancer 2020 Jul;11(7):1848-1860
Thoracic cancer 2020 Jul;11(7):1848-1860
Astragaloside IV protects against hyperglycemia-induced vascular endothelial dysfunction by inhibiting oxidative stress and Calpain-1 activation.
Nie Q, Zhu L, Zhang L, Leng B, Wang H
Life sciences 2019 Sep 1;232:116662
Life sciences 2019 Sep 1;232:116662
Suppressing the Na(+)/H(+) exchanger 1: a new sight to treat depression.
Deng X, Ji Z, Xu B, Guo L, Xu L, Qin T, Feng L, Ma Z, Fu Q, Qu R, Quo Q, Ma S
Cell death & disease 2019 May 8;10(5):370
Cell death & disease 2019 May 8;10(5):370
Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.
Wang S, Huang Y, Yan Y, Zhou H, Wang M, Liao L, Wang Z, Chen D, Ji D, Xia X, Liu F, Huang J, Xiong K
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2019 Jan;221:57-67
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2019 Jan;221:57-67
Triptolide protects against TGF-β1-induced pulmonary fibrosis by regulating FAK/calpain signaling.
Zhang P, Liu J, Zong R
Experimental and therapeutic medicine 2019 Dec;18(6):4781-4789
Experimental and therapeutic medicine 2019 Dec;18(6):4781-4789
Expression of Cell Cycle-related Proteins p16, p27 and Ki-67 Proliferating Marker in Laryngeal Squamous Cell Carcinomas and in Laryngeal Papillomas.
Ciesielska U, Zatonski T, Nowinska K, Ratajczak-Wielgomas K, Grzegrzolka J, Piotrowska A, Olbromski M, Pula B, Podhorska-Okolow M, Dziegiel P
Anticancer research 2017 May;37(5):2407-2415
Anticancer research 2017 May;37(5):2407-2415
Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.
Zhang J, Lu S, Zhou Y, Meng K, Chen Z, Cui Y, Shi Y, Wang T, He QY
Proteomics 2017 Jul;17(13-14)
Proteomics 2017 Jul;17(13-14)
Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.
Kmiecik AM, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski M, Podhorska-Okolow M, Dziegiel P
PloS one 2015;10(5):e0124865
PloS one 2015;10(5):e0124865
Calpain Cleaves Most Components in the Multiple Aminoacyl-tRNA Synthetase Complex and Affects Their Functions.
Lei HY, Zhou XL, Ruan ZR, Sun WC, Eriani G, Wang ED
The Journal of biological chemistry 2015 Oct 23;290(43):26314-27
The Journal of biological chemistry 2015 Oct 23;290(43):26314-27
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP(CAPN1 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using CAPN1 antibody 10538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 10538-1-AP(CAPN1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 10538-1-AP(CAPN1 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue