Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- PA1364 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Calpain 1/CAPN1 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CALPAIN 1/CAPN1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IHC-F, ICC, ICC/IF. Reactive species: Human;Mouse;Rat. CALPAIN 1/CAPN1 information: Molecular Weight: 81890 MW; Subcellular Localization: Cytoplasm . Cell membrane . Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719); Tissue Specificity: Ubiquitous.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling.
Protective effect of donepezil hydrochloride on cerebral ischemia/reperfusion injury in mice.
Suppression of NHE1 by small interfering RNA inhibits HIF-1α-induced angiogenesis in vitro via modulation of calpain activity.
Ye T, Wang Q, Zhang Y, Song X, Yang D, Li D, Li D, Su L, Yang Y, Ma S
PloS one 2015;10(3):e0120178
PloS one 2015;10(3):e0120178
Protective effect of donepezil hydrochloride on cerebral ischemia/reperfusion injury in mice.
Wang T, Lv P, Jin W, Zhang H, Lang J, Fan M
Molecular medicine reports 2014 Feb;9(2):509-14
Molecular medicine reports 2014 Feb;9(2):509-14
Suppression of NHE1 by small interfering RNA inhibits HIF-1α-induced angiogenesis in vitro via modulation of calpain activity.
Mo XG, Chen QW, Li XS, Zheng MM, Ke DZ, Deng W, Li GQ, Jiang J, Wu ZQ, Wang L, Wang P, Yang Y, Cao GY
Microvascular research 2011 Mar;81(2):160-8
Microvascular research 2011 Mar;81(2):160-8
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Calpain 1 using anti-Calpain 1 antibody (PA1364). Calpain 1 was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calpain 1 Antibody (PA1364) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image