LS-C815526

antibody from LSBio

Targeting: AP1B1 ADTB1, AP105A, BAM22, CLAPB2

Western blot (Data presented on provider website)

Immunoprecipitation (Enhanced validation)

Antibody Data

Product number

LS-C815526

Provider

LSBio

Product name

AP1B1 / BAM22 Antibody (Preservative Free) LS-C815526

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

AP1B1 was immunoprecipitated using: Lane A: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate. Lane C:0.5 mg Hela Whole Cell Lysate. 1 uL anti-AP1B1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-AP1B1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 105 kDa. Observed band size: 112 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

AP1B1 was immunoprecipitated using: Lane A: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate. Lane C:0.5 mg Hela Whole Cell Lysate. 1 uL anti-AP1B1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-AP1B1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 105 kDa. Observed band size: 112 kDa.