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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00007172-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007172-M01, RRID:AB_436976
- Product name
- TPMT monoclonal antibody (M01), clone 1B5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant TPMT.
- Antigen sequence
MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVN
GKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLC
GKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNL
SYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPR
TNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLG
KKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGK
ICNIRRLEKVDAFEERHKSWGIDCLFEKLYLLTEK- Isotype
- IgG
- Antibody clone number
- 1B5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references S-adenosylmethionine regulates thiopurine methyltransferase activity and decreases 6-mercaptopurine cytotoxicity in MOLT lymphoblasts.
A novel gene delivery system for stable transfection of thiopurine-S-methyltransferase gene in versatile cell types.
Milek M, Karas Kuzelicki N, Smid A, Mlinaric-Rascan I
Biochemical pharmacology 2009 Jun 15;77(12):1845-53
Biochemical pharmacology 2009 Jun 15;77(12):1845-53
A novel gene delivery system for stable transfection of thiopurine-S-methyltransferase gene in versatile cell types.
Egle R, Milek M, Mlinaric-Rascan I, Fahr A, Kristl J
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2008 May;69(1):23-30
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2008 May;69(1):23-30
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TPMT monoclonal antibody (M01), clone 1B5 Western Blot analysis of TPMT expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of TPMT expression in transfected 293T cell line by TPMT monoclonal antibody (M01), clone 1B5.Lane 1: TPMT transfected lysate(28.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to TPMT on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to TPMT on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
