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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-23551 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCARA5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The scavenger receptor SCARA5 is an endocytic receptor for von Willebrand factor expressed by littoral cells in the human spleen.
Swystun LL, Ogiwara K, Lai JD, Ojala JRM, Rawley O, Lassalle F, Notley C, Rengby O, Michels A, Nesbitt K, Tryggvason K, Lillicrap D
Journal of thrombosis and haemostasis : JTH 2019 Aug;17(8):1384-1396
Journal of thrombosis and haemostasis : JTH 2019 Aug;17(8):1384-1396
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SCARA5 in various lysates. Samples were incubated with SCARA5 polyclonal antibody (Product # PA5-23551) using a dilution of 1:500 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Mouse heart tissue lysate; Lane 2: T47D whole cell lysate. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SCARA5 in mouse heart tissue lysates. Samples were incubated with SCARA5 polyclonal antibody (Product # PA5-23551). Lysates at 15 µg/lane. SCAR5 protein (arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SCARA5 in formalin fixed and paraffin embedded human skeletal muscle. Samples were incubated with SCARA5 polyclonal antibody (Product # PA5-23551) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
