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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-30793 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CIDEC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2. Predicted reactivity: Pig (81%), Sheep (84%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Human CIDEC transgene improves lipid metabolism and protects against high-fat diet-induced glucose intolerance in mice.
Fat-Specific Protein 27 Regulation of Vascular Function in Human Obesity.
Gupta A, Balakrishnan B, Karki S, Slayton M, Jash S, Banerjee S, Grahn THM, Jambunathan S, Disney S, Hussein H, Kong D, Lowell BB, Natarajan P, Reddy UK, Gokce N, Sharma VM, Puri V
The Journal of biological chemistry 2022 Sep;298(9):102347
The Journal of biological chemistry 2022 Sep;298(9):102347
Fat-Specific Protein 27 Regulation of Vascular Function in Human Obesity.
Karki S, Farb MG, Sharma VM, Jash S, Zizza EJ, Hess DT, Carmine B, Carter CO, Pernar LI, Apovian CM, Puri V, Gokce N
Journal of the American Heart Association 2019 Jun 4;8(11):e011431
Journal of the American Heart Association 2019 Jun 4;8(11):e011431
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CIDEC Polyclonal Antibody detects CIDEC protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CIDEC protein stained by CIDEC Polyclonal Antibody (Product # PA5-30793) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Comparison of FSP 27 (fat-specific protein 27) protein in primary endothelial cells from subcutaneous vs visceral fat of obese subjects. A , Representative immunofluorescence image of isolated endothelial cells demonstrating significantly reduced expression of FSP 27 protein (red= FSP 27, green= CD 31, endothelial cell marker, blue=DAPI, a nuclear stain) in endothelial cells isolated from visceral compared with subcutaneous depots from obese subjects. B , Quantification of FSP 27 protein expression in endothelial cells isolated from subcutaneous and visceral fat (n=14, P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Interaction of FSP 27 and VEGF -A. A , Co-immunoprecipitation of FSP 27 followed by immunoblot of VEGF -A in cultured human aortic endothelial cells demonstrating an interaction between FSP 27 and VEGF -A. B , In-vitro transcription translation of FSP 27 and VEGF -A followed by immunoblot of FSP 27 depicting an interaction between FSP 27 and VEGF -A. C , Western blot showing that si RNA (small interfering RNA )-mediated inhibition of FSP 27 nearly fully abolished VEGF -A in human aortic endothelial cells. Experiments were repeated 3 times. FSP27 indicates fat-specific protein 27; IP, immunoprecipitation; IB, immunoblot; VEFG-A, vascular endothelial growth factor-A.
