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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [2]
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- Product number
- LS-C98881 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C98881, RRID:AB_2210100
- Product name
- TRP32 / TXNL1 Antibody (aa256-289) LS-C98881
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining. The results correspond precisely to TrxL-1 mRNA expression shown with in situ hybridization (Jimenez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization. The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen. Data courtesy of Prof. Markku Pelto-Huikko, Department of Developmental Biology, Medical School,Tampere University, Finland.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining. The results correspond precisely to TrxL-1 mRNA expression shown with in situ hybridization (Jimenez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization. The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen. Data courtesy of Prof. Markku Pelto-Huikko, Department of Developmental Biology, Medical School,Tampere University, Finland.
