Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-11420 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-TXNL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse and rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. A TXNL1 polyclonal antibody (Product # PA5-11420) shows strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining. The results correspond precisely to TrxL-1 mRNA expression shown with in situ hybridization and could be abolished with peptide used for immunization (Blocking peptide). The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion.