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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP50279PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP50279PU-N, RRID:AB_11147903
- Product name
- anti ASXL1 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 528-556 amino acids from the Central region of human ASXL1
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in mouse liver tissue lysates (35ug/lane) using ASXL1 antibody (Center) Cat.-No AP50279PU-N.This demonstrates the ASXL1 antibody detected the ASXL1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ASXL1 Antibody (Center) Cat.-No AP50279PU-N with Hela cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue using ASXL1 Antibody Cat.-No AP50279PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of Hela cells (Right Histogram) using ASXL1 antibody (Center) Cat.-No AP50279PU-N compared to a negative control cell (Left Histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
