PA5-78646

antibody from Invitrogen Antibodies

Targeting: ASXL1 KIAA0978

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78646

Provider

Invitrogen Antibodies

Product name

ASXL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: human ASXL1-transfected 293T cells Predicted Reactivity: Mouse (93%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASXL1 in A) 293T whole cell lysate, B) whole cell lysate of GFP-human ASXL1-transfected 293T cells using 50 µg of protein. Sample was incubated with ASXL1 polyclonal antibody (Product # PA5-78646) using a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ASXL1 Polyclonal Antibody detects ASXL1 protein by western blot analysis. A. 50 µg 293T whole cell lysate/extract. B. 50 µg whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells. ASXL1 Polyclonal Antibody (Product # PA5-78646) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ASXL1 Polyclonal Antibody detects ASXL1 protein at nucleus in human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma. ASXL1 Polyclonal Antibody (Product # PA5-78646) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.