Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-78646 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASXL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: human ASXL1-transfected 293T cells
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ASXL1 in A) 293T whole cell lysate, B) whole cell lysate of GFP-human ASXL1-transfected 293T cells using 50 µg of protein. Sample was incubated with ASXL1 polyclonal antibody (Product # PA5-78646) using a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ASXL1 Polyclonal Antibody detects ASXL1 protein by western blot analysis. A. 50 µg 293T whole cell lysate/extract. B. 50 µg whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells. ASXL1 Polyclonal Antibody (Product # PA5-78646) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ASXL1 Polyclonal Antibody detects ASXL1 protein at nucleus in human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma. ASXL1 Polyclonal Antibody (Product # PA5-78646) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
