Explore
Validate
Learn
Immunocytochemistry
Immunohistochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-29768 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Orphanin FQ Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- PA1-29768 detects Orphanin FQ from non-human primate, rat samples. PA1-29768 has been successfully used in immunohistochemistry applications. The PA1-29768 immunogen is: FGGFTGARKSARKLANQ Corresponding to residues 122-138 of rat Orphanin FQ Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- Lot Dependent
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Orphanin FQ using an Orphanin FQ polyclonal antibody (Product # PA1-29768). Confocal images showing OFQ (red), GnRH (green) and their colocalization (yellow) in the ovine preoptic area and hypothalamus. A-C: Low power view of the preoptic area at the level of the organum vasculosum of the lamina terminalis (OVLT) showing OFQ and GnRH cells, and their colocalization (e.g., white arrows). Bar = 100 µm. D-F: High power images of a double-labeled OFQ/GnRH cell; note that the immunofluorescence for OFQ has a punctateappearance in the cytoplasm as opposed to the more diffuse appearance of the GnRH signal. Bar 26 = 20 µm. G-I: Single-labeled OFQ cells (e.g., red arrow) in the arcuate nucleus. Note that many GnRH fibers coursing through this region are also OFQ-positive (e.g., white arrows). 3v = third ventricle. Bar = 100 µm.
