Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-20389 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TBC1D1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is Daudi cell lysate. PA5-20389 can be used with blocking peptide PEP-0506.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Daudi cell lysate using a TBC1D1 polyclonal antibody (Product # PA5-20389) at (A) 1, (B) 2 and (C) 4 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TBC1D1 expression in (A) 293 and (B) Daudi cell lysate with TBC1D1 Polyclonal Antibody (Product # PA5-20389) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Ramos (Lane 1), Daudi (Lane 2) and MOLT-4 (Lane 3). The blot was probed with TBC1D1 Polyclonal Antibody (Product # PA5-20389, 1µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A band at 150 kDa corresponding to TBC1D1 was observed across the cell lines tested.