PAB29365

antibody from Abnova Corporation

Targeting: NEK9 DKFZp434D0935, MGC16714, Nek8, NERCC, NERCC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29365

Provider

Abnova Corporation

Product name

NEK9 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human NEK9.

Antigen sequence

VSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTER
PHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIV
EKVLNSKTIRSNSSGLSIGTVFQSSSPGGGGGGGG
GEEEDSQQESETPDPSGGFRGTMEADRGMEGLISP
TEAM


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line EFO-21 Lane 3: Human cell line A-431 with NEK9 polyclonal antibody (Cat # PAB29365) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with NEK9 polyclonal antibody (Cat # PAB29365) at 1-4 ug/mL concentration shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with NEK9 polyclonal antibody (Cat # PAB29365) shows cytoplasmic positivity in cells of seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)