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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- PA5-28173 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cdc34 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji.
- Concentration
- 0.61 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cdc34 Polyclonal Antibody (Product # PA5-28173). Sample (30 µg whole cell lysate). A: Raji. 12% SDS PAGE. Cdc34 Polyclonal Antibody (Product # PA5-28173) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CDC34 in paraformaldehyde-fixed A549 cells using a CDC34 polyclonal antibody (Product # PA5-28173) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Cdc34 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Cdc34 Polyclonal Antibody (Product # PA5-28173) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
