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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- 703803 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GALR1 Recombinant Rabbit Monoclonal Antibody (18H2L18)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 18H2L18
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells transiently transfected with GALR1 were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-GALR1 Recombinant Rabbit Monoclonal Antibody (Product # 703803, 1:500 dilution). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using an immunofluorescence microscope. Note, strong staining of transfected cells.
