H00003400-M13

antibody from Abnova Corporation

Targeting: ID4 bHLHb27

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003400-M13

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003400-M13, RRID:AB_10905968

Product name

ID4 monoclonal antibody (M13), clone 3F11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant ID4.

Antigen sequence

MKAVSPVRPSGRKAPSGCGGGELALRCLAEHGHSL
GGSAAAAAAAAAARCKAAEAAADEPALCLQCDMND
CYSRLRRLVPTIPPNKKVSKVEILQHVIDYILDLQ
LALETHPALLRQPPPPAPPHHPAGTCPAAPPRTPL
TALNTDPAGAVNKQGDSILCR


Isotype

IgG

Antibody clone number

3F11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ID4 expression in transfected 293T cell line by ID4 monoclonal antibody (M13), clone 3F11.Lane 1: ID4 transfected lysate (Predicted MW: 16.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ID4 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol