PA5-30012

antibody from Invitrogen Antibodies

Targeting: ECH1 HPXEL

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30012

Provider

Invitrogen Antibodies

Product name

ECH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A549, HepG2, mouse brain. Predicted reactivity: Mouse (80%), Rat (82%), Pig (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.71 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

RNA Sequence Analyses throughout the Course of Mouse Cardiac Laminopathy Identify Differentially Expressed Genes for Cell Cycle Control and Mitochondrial Function.

Shao Z, Koh W, Ni Y, Li W, Agatisa-Boyle B, Merkurjev D, Tang WHW
Scientific reports 2020 Apr 20;10(1):6632

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Analysis of the effect of a novel therapeutic for type 2 diabetes on the proteome of a muscle cell line.

Young PA, Leonard S, Martin DS, Findlay JB
Proteomics 2016 Jan;16(1):70-9

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ECH1 in methanol-fixed HeLa cells using an ECH1 polyclonal antibody (Product # PA5-30012) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Secondary validation of some of the changes observed in response to RTC-15 treatment. Western blotting analysis of (A) TPI, (B) annexin A2, (C) calumenin, (D) ZPR1, and (E) ECH1 expression. All experiments were conducted in triplicate. beta-Actin was used as a loading control where appropriate. As no phosphospecific antibodies were available for RCN-1, the protein was first immunoprecipitated and subsequently subjected to Western blotting analysis. Serine phosphorylation was assessed using anti-phosphoserine antibody and total protein levels were observed using anti-RCN-1 antibody (F). Change in ACC phosphorylation in response to RTC-15 (G).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Validation of One-month-old Mouse Heart RNA-Sequencing Results by qRT-PCR and Western Blot. Samples (RNAs and proteins) being analyzed were extracted from WT and Lmna -/- mouse heart tissues at 1 month of age. ( a ) RT-qPCR validation of down-regulated genes ( ACDVL, ATP5G1, CRAT, ECH1, KCDN2, P2RY1, PPARA ) identified by RNA-Sequencing. The same Ct method was used for analysis as described in Fig. 3 legends. The relative expression levels of all the listed genes are showed as ""mean +- SEM"" on Y axis. The expression levels of all the listed genes among 1-month Lmna -/- mouse hearts were significantly lower than the levels among 1-month WT mouse hearts (p < 0.05). ( b ) RT-qPCR validation of up-regulated genes ( DUSP4, LOX, FHL1, MYOM2, NMRK2 ) identified by RNA-Sequencing. The same Ct method was used for analysis. The relative expression levels of all the listed genes are showed as ""mean +- SEM"" on Y axis. The expression levels in 1-month Lmna -/- mouse hearts were significantly higher than those in 1-month WT mouse hearts for all the genes (p < 0.05). ( c ) Western blot validation of protein expressions of representative down-regulated ( ECH1 and PPARA ) and up-regulated ( DUSP4 and FHL1 ) genes. beta-actin (for ECH1 and PPARA ) or GAPDH (for DUSP4 and FHL1 ) was used as a loading control. Three repeated experiments were conducted for each protein with similar results showed in the figure.