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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-30012 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ECH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A549, HepG2, mouse brain.
- Concentration
- 0.71 mg/mL
Submitted references RNA Sequence Analyses throughout the Course of Mouse Cardiac Laminopathy Identify Differentially Expressed Genes for Cell Cycle Control and Mitochondrial Function.
Analysis of the effect of a novel therapeutic for type 2 diabetes on the proteome of a muscle cell line.
Shao Z, Koh W, Ni Y, Li W, Agatisa-Boyle B, Merkurjev D, Tang WHW
Scientific reports 2020 Apr 20;10(1):6632
Scientific reports 2020 Apr 20;10(1):6632
Analysis of the effect of a novel therapeutic for type 2 diabetes on the proteome of a muscle cell line.
Young PA, Leonard S, Martin DS, Findlay JB
Proteomics 2016 Jan;16(1):70-9
Proteomics 2016 Jan;16(1):70-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ECH1 Polyclonal Antibody (Product # PA5-30012). Sample (30 µg of whole cell lysate). Lane A: A549. 10% SDS PAGE. ECH1 Polyclonal Antibody . ECH1 Polyclonal Antibody (Product # PA5-30012) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ECH1 Polyclonal Antibody (Product # PA5-30012). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. ECH1 Polyclonal Antibody (Product # PA5-30012) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ECH1 in methanol-fixed HeLa cells using an ECH1 polyclonal antibody (Product # PA5-30012) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ECH1 Polyclonal Antibody detects ECH1 protein at mitochondrion on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. ECH1 Polyclonal Antibody (Product # PA5-30012) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Secondary validation of some of the changes observed in response to RTC-15 treatment. Western blotting analysis of (A) TPI, (B) annexin A2, (C) calumenin, (D) ZPR1, and (E) ECH1 expression. All experiments were conducted in triplicate. beta-Actin was used as a loading control where appropriate. As no phosphospecific antibodies were available for RCN-1, the protein was first immunoprecipitated and subsequently subjected to Western blotting analysis. Serine phosphorylation was assessed using anti-phosphoserine antibody and total protein levels were observed using anti-RCN-1 antibody (F). Change in ACC phosphorylation in response to RTC-15 (G).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Validation of One-month-old Mouse Heart RNA-Sequencing Results by qRT-PCR and Western Blot. Samples (RNAs and proteins) being analyzed were extracted from WT and Lmna -/- mouse heart tissues at 1 month of age. ( a ) RT-qPCR validation of down-regulated genes ( ACDVL, ATP5G1, CRAT, ECH1, KCDN2, P2RY1, PPARA ) identified by RNA-Sequencing. The same Ct method was used for analysis as described in Fig. 3 legends. The relative expression levels of all the listed genes are showed as ""mean +- SEM"" on Y axis. The expression levels of all the listed genes among 1-month Lmna -/- mouse hearts were significantly lower than the levels among 1-month WT mouse hearts (p < 0.05). ( b ) RT-qPCR validation of up-regulated genes ( DUSP4, LOX, FHL1, MYOM2, NMRK2 ) identified by RNA-Sequencing. The same Ct method was used for analysis. The relative expression levels of all the listed genes are showed as ""mean +- SEM"" on Y axis. The expression levels in 1-month Lmna -/- mouse hearts were significantly higher than those in 1-month WT mouse hearts for all the genes (p < 0.05). ( c ) Western blot validation of protein expressions of representative down-regulated ( ECH1 and PPARA ) and up-regulated ( DUSP4 and FHL1 ) genes. beta-actin (for ECH1 and PPARA ) or GAPDH (for DUSP4 and FHL1 ) was used as a loading control. Three repeated experiments were conducted for each protein with similar results showed in the figure.
