PA5-20066

antibody from Invitrogen Antibodies

Targeting: BIRC2 API1, c-IAP1, cIAP1, hiap-2, MIHB, RNF48

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20066

Provider

Invitrogen Antibodies

Product name

cIAP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects both c-IAP1 and c-IAP2. A suggested positive control is human lung tissue lysate. PA5-20066 can be used with blocking peptide PEP-0185.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human lung lysate using a cIAP polyclonal antibody (Product # PA5-20066) at 1 (lane A), 2 (lane B), and 4 (lane C) µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human and Rat Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: cIAP1 Polyclonal Antibody (Product # PA5-20066) (1 µg/mL ), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: cIAP1 Polyclonal Antibody (Product # PA5-20066) (2 µg/mL ), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human Lung Lysate. Loading: 15 µg of lysates per lane. Antibodies: cIAP1 Polyclonal Antibody (Product # PA5-20066) (A: 1 µg/mL, B: 2 µg/mL and C: 4 µg/mL ), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue abeling cIAP with cIAP1 Polyclonal Antibody (Product # PA5-20066) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung tissue using cIAP1 Polyclonal Antibody (Product # PA5-20066) at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.