PA5-29085

antibody from Invitrogen Antibodies

Targeting: BIRC2 API1, c-IAP1, cIAP1, hiap-2, MIHB, RNF48

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29085

Provider

Invitrogen Antibodies

Product name

cIAP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NCI-H929, Jurkat, rat muscle, Mouse B cell, Mouse T cell, Mouse Thymocyte.

Concentration

0.56 mg/mL

References

Submitted references

XPO1 inhibitor combination therapy with bortezomib or carfilzomib induces nuclear localization of IκBα and overcomes acquired proteasome inhibitor resistance in human multiple myeloma.

Turner JG, Kashyap T, Dawson JL, Gomez J, Bauer AA, Grant S, Dai Y, Shain KH, Meads M, Landesman Y, Sullivan DM
Oncotarget 2016 Nov 29;7(48):78896-78909

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of cIAP1 using 30 µg of Jurkat lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a cIAP1 polyclonal antibody (Product # PA5-29085) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of cIAP1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a cIAP1 Polyclonal Antibody (Product # PA5-29085) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using cIAP1 Polyclonal Antibody (Product # PA5-29085). Sample (50 µg of whole cell lysate). Lane A: Rat muscle. 7.5% SDS PAGE. cIAP1 Polyclonal Antibody (Product # PA5-29085) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using cIAP1 Polyclonal Antibody (Product # PA5-29085). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 Polyclonal Antibody (Product # PA5-29085) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), HCT 116 (Lane 2), Hep G2 (Lane 3), NIH-3T3 (Lane 4), tissue extracts of Mouse Skeletal Muscle (Lane 5) and Mouse Lung (Lane 6). The blot was probed with Anti-cIAP1 Polyclonal Antibody (Product # PA5-29085, 1µg/ml dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 70 kDa band corresponding to cIAP1 was observed across all cell lines and tissues tested. A non specific band around 30 kDa was also observed in all cell lines.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

cIAP1 Polyclonal Antibody detects cIAP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. cIAP1 stained by cIAP1 Polyclonal Antibody (Product # PA5-29085) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

cIAP1 Polyclonal Antibody detects cIAP1 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CIAP1 Polyclonal Antibody (Product # PA5-29085) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.