Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 15296-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15296-1-AP, RRID:AB_2068455
- Product name
- CARS antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CARS antibody (Cat. #15296-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies.
CARS senses cysteine deprivation to activate AMPK for cell survival.
Vulsteke JB, Derua R, Dubucquoi S, Coutant F, Sanges S, Goncalves D, Wuyts G, De Haes P, Blockmans D, Wuyts WA, Claeys KG, De Langhe E, Fabien N, Bossuyt X
Annals of the rheumatic diseases 2023 Apr;82(4):546-555
Annals of the rheumatic diseases 2023 Apr;82(4):546-555
CARS senses cysteine deprivation to activate AMPK for cell survival.
Yuan M, Yan R, Zhang Y, Qiu Y, Jiang Z, Liu H, Wang Y, Sun L, Zhang H, Gao P
The EMBO journal 2021 Nov 2;40(21):e108028
The EMBO journal 2021 Nov 2;40(21):e108028
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15296-1-AP(CARS antibody) at dilution of 1:1500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using CARS antibody 15296-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lung cancer using 15296-1-AP(CARS antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lung cancer using 15296-1-AP(CARS antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue