PAB28665

antibody from Abnova Corporation

Targeting: CARS1 CARS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28665

Provider

Abnova Corporation

Product name

CARS polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CARS.

Antigen sequence

HLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETT
DPDKKQMLERIQHAVQLATEPLEKAVQSRLTGEEV
NSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSK
LPKFWEGDFHRDMEALNVLPPDVL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251MG sp, Lane 3: A-431 with CARS polyclonal antibody (Cat # PAB28665) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with CARS polyclonal antibody (Cat # PAB28665) at 1-4 ug/mL shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with CARS polyclonal antibody (Cat # PAB28665) shows cytoplasmic positivity in exocrine glandular cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)