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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB9345-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IFN-alpha 2/IFNA2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IFN-alpha 2/IFNA2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IFNE, rhIFNB, rhIFNG, or recombinant cotton rat IFNA is observed. In sandwich ELISAs, less than 8% cross-reactivity with rhIFNA-7, rhIFNA-8, and rhIFNA-10 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01563- Isotype
- IgG
- Antibody clone number
- 967314
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IFN-alpha 2/IFNA2 in HeLa Human Cell Line. IFN-alpha 2/IFNA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human IFN-alpha 2/IFNA2 Monoclonal Antibody (Catalog # MAB9345) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
