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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- PA5-119649 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFN alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Mouse liver tissue lysate, N2A, SHG-44, SH-SY5Y, rat brain tissue, human kidney tissue, mouse brain tissue.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFNA1 on mouse liver tissue lysate using IFN alpha Polyclonal Antibody (Product # PA5-119649).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IFNA1 in N2A cells (green) using IFN alpha Polyclonal Antibody (Product # PA5-119649). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IFNA1 in SHG-44 cells (green) using IFN alpha Polyclonal Antibody (Product # PA5-119649). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IFNA1 in SH-SY5Y cells (green) using IFN alpha Polyclonal Antibody (Product # PA5-119649). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat brain tissue using IFN alpha Polyclonal Antibody (Product # PA5-119649). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded human kidney tissue using IFN alpha Polyclonal Antibody (Product # PA5-119649). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse brain tissue tissue using IFN alpha Polyclonal Antibody (Product # PA5-119649). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of IFN alpha in SH-SY5Y cells using IFN alpha Polyclonal Antibody (Product # PA5-119649) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
