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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-30621 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HCT116.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KLF3 using 30 µg of HCT116 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a KLF3 polyclonal antibody (Product # PA5-30621) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using KLF3 Polyclonal Antibody (Product # PA5-30621). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF3 Polyclonal Antibody (Product # PA5-30621) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using KLF3 (Product # PA5-30621) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
