sc-12739

antibody from Santa Cruz Biotechnology

Targeting: CSNK2B Ckb1, Ckb2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-12739

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-12739, RRID:AB_626792

Product name

Anti-CSNK2B

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

Three novel downstream promoter elements regulate MHC class I promoter activity in mammalian cells.

Lee N, Iyer SS, Mu J, Weissman JD, Ohali A, Howcroft TK, Lewis BA, Singer DS
PloS one 2010 Dec 13;5(12):e15278

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Arsenic-induced malignant transformation of human keratinocytes: involvement of Nrf2.

Pi J, Diwan BA, Sun Y, Liu J, Qu W, He Y, Styblo M, Waalkes MP
Free radical biology & medicine 2008 Sep 1;45(5):651-8

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Mechanistic studies of the mitotic activation of Mos.

Yue J, Ferrell JE Jr
Molecular and cellular biology 2006 Jul;26(14):5300-9

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 27

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic as well as nuclear positivity in neuronal cells and glial cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.