Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-2909-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD290 (TLR10) Monoclonal Antibody (3C10C5), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 3C10C5 monoclonal antibody reacts with human CD290, which is also known as Toll-like receptor 10 (TLR10). This type I transmembrane glycoprotein is expressed on B cells, monocytes, regulatory T cells (Tregs), and plasmacytoid dendritic cells. Moreover, CD290 expression is dependent on Foxp3 and NFAT in Tregs. Studies demonstrate that CD290 can self-dimerize, as well as heterodimerize with other members of the TLR family such as TLR1 and TLR2. Ligand binding results in association of the receptor with the adapter molecule MyD88 and subsequent activation of signaling pathways. Although the ligands of CD290 remain undefined, this receptor has been associated with the immune response against microbial and fungal infection and autoimmune diseases such as Crohn's disease and systemic lupus erythematosous. Applications Reported: This 3C10C5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3C10C5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 3C10C5
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Toll-like receptor 10 controls TLR2-induced cytokine production in monocytes from patients with Parkinson's disease.
Increased Expression of the Innate Immune Receptor TLR10 in Obesity and Type-2 Diabetes: Association with ROS-Mediated Oxidative Stress.
Molecular modeling-based evaluation of hTLR10 and identification of potential ligands in Toll-like receptor signaling.
Human TLRs 10 and 1 share common mechanisms of innate immune sensing but not signaling.
FOXP3 regulates TLR10 expression in human T regulatory cells.
Human TLR10 is a functional receptor, expressed by B cells and plasmacytoid dendritic cells, which activates gene transcription through MyD88.
da Rocha Sobrinho HM, Saar Gomes R, da Silva DJ, Quixabeira VBL, Joosten LAB, Ribeiro de Barros Cardoso C, Ribeiro-Dias F
Journal of neuroscience research 2021 Oct;99(10):2511-2524
Journal of neuroscience research 2021 Oct;99(10):2511-2524
Increased Expression of the Innate Immune Receptor TLR10 in Obesity and Type-2 Diabetes: Association with ROS-Mediated Oxidative Stress.
Sindhu S, Akhter N, Kochumon S, Thomas R, Wilson A, Shenouda S, Tuomilehto J, Ahmad R
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(2):572-590
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(2):572-590
Molecular modeling-based evaluation of hTLR10 and identification of potential ligands in Toll-like receptor signaling.
Govindaraj RG, Manavalan B, Lee G, Choi S
PloS one 2010 Sep 16;5(9):e12713
PloS one 2010 Sep 16;5(9):e12713
Human TLRs 10 and 1 share common mechanisms of innate immune sensing but not signaling.
Guan Y, Ranoa DR, Jiang S, Mutha SK, Li X, Baudry J, Tapping RI
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5094-103
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5094-103
FOXP3 regulates TLR10 expression in human T regulatory cells.
Bell MP, Svingen PA, Rahman MK, Xiong Y, Faubion WA Jr
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 1;179(3):1893-900
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 1;179(3):1893-900
Human TLR10 is a functional receptor, expressed by B cells and plasmacytoid dendritic cells, which activates gene transcription through MyD88.
Hasan U, Chaffois C, Gaillard C, Saulnier V, Merck E, Tancredi S, Guiet C, Brière F, Vlach J, Lebecque S, Trinchieri G, Bates EE
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):2942-50
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):2942-50
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD290 (TLR10) PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye