Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-9911-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD281 (TLR1) Monoclonal Antibody (GD2.F4), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The GD2.F4 antibody reacts with human Toll-like receptor 1 (TLR1). TLR1 is expressed in low numbers on the surface of peripheral blood monocytes and even lower numbers on dendritic cells. A very high degree of donor variability has been reported for TLR1 expression. eBioscience manufactured GD2.F4 mAb has been tested by flow cytometry, in parallel with a sample from the developer using cells from healthy donors. eBioscience and the reference antibody gave similar results: donors tested confirmed low levels expression of TLR1 on PBMC (less than 1/4 log shift). While the ligand and exact function of TLR1 are not defined yet, it has been suggested that TLR1 may cooperate with TLR2 in response to certain antagonists. Despite low levels of surface expression as detected by flow cytometric analysis, TLRs are efficient signal transducing molecules in monocytes and dendritic cells. To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. Applications Reported: The GD2.F4 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. To date, applicability of this mAb for IP, WB, or inhibition of function has not been reported. Applications Tested: The GD2.F4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GD2.F4
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Toll-like receptors 1 and 2 cooperatively mediate immune responses to curli, a common amyloid from enterobacterial biofilms.
Human TLRs 10 and 1 share common mechanisms of innate immune sensing but not signaling.
Activation and regulation of Toll-like receptors 2 and 1 in human leprosy.
Regulation of Toll-like receptors in human monocytes and dendritic cells.
Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses.
Tükel C, Nishimori JH, Wilson RP, Winter MG, Keestra AM, van Putten JP, Bäumler AJ
Cellular microbiology 2010 Oct;12(10):1495-505
Cellular microbiology 2010 Oct;12(10):1495-505
Human TLRs 10 and 1 share common mechanisms of innate immune sensing but not signaling.
Guan Y, Ranoa DR, Jiang S, Mutha SK, Li X, Baudry J, Tapping RI
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5094-103
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5094-103
Activation and regulation of Toll-like receptors 2 and 1 in human leprosy.
Krutzik SR, Ochoa MT, Sieling PA, Uematsu S, Ng YW, Legaspi A, Liu PT, Cole ST, Godowski PJ, Maeda Y, Sarno EN, Norgard MV, Brennan PJ, Akira S, Rea TH, Modlin RL
Nature medicine 2003 May;9(5):525-32
Nature medicine 2003 May;9(5):525-32
Regulation of Toll-like receptors in human monocytes and dendritic cells.
Visintin A, Mazzoni A, Spitzer JH, Wyllie DH, Dower SK, Segal DM
Journal of immunology (Baltimore, Md. : 1950) 2001 Jan 1;166(1):249-55
Journal of immunology (Baltimore, Md. : 1950) 2001 Jan 1;166(1):249-55
Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses.
Wyllie DH, Kiss-Toth E, Visintin A, Smith SC, Boussouf S, Segal DM, Duff GW, Dower SK
Journal of immunology (Baltimore, Md. : 1950) 2000 Dec 15;165(12):7125-32
Journal of immunology (Baltimore, Md. : 1950) 2000 Dec 15;165(12):7125-32
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD281 (TLR1) Purified vs. Purified Mouse IgG1 Isotype Control (Product # 14-4714-82). Cell sin the monocyte gate were used for analysis.