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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-88037 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMD14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FRLINANMMV LGHEPRQTTS NLGHLNKPSI QALIHGLNRH YYSITINYRK NELEQKMLLN LHKKSWMEGL TLQDYSEHCK HNESVVKEML ELAKNYNKAV EEEDKMTPEQ LAIKNVGKQD PKRHLEEHVD VLMTSNIVQC L; Positive Samples: HepG2, HeLa, A-549, 293T, Raji, Mouse heart, Mouse kidney, Mouse liver
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.37 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using PSMD14 Polyclonal antibody (Product # PA5-88037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PSMD14 was performed in NIH-3T3 cells using PSMD14 Polyclonal Antibody (Product # PA5-88037) at a dilution of 1:100. Blue: DAPI for nuclear staining.
