Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AFT-001-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC19A1/RFC1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat small intestine lysate (lanes 3 and 7) and rat liver membranes (lanes 4 and 8): - 1-4. Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (1:400). 5-8. Anti-SLC19A1/RFC1 (extracellular) Antibody , preincubated with SLC19A1/RFC1 (extracellular) Blocking Peptide (#BLP-FT001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat small intestine lysate (lanes 3 and 7) and rat liver membranes (lanes 4 and 8): - 1-4. Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (1:400). 5-8. Anti-SLC19A1/RFC1 (extracellular) Antibody , preincubated with SLC19A1/RFC1 (extracellular) Blocking Peptide (#BLP-FT001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 3) and human K562 myelogenous leukemia cell line lysate (lanes 2 and 4): - 1, 2. Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (1:400). 3, 4. Anti-SLC19A1/RFC1 (extracellular) Antibody , preincubated with SLC19A1/RFC1 (extracellular) Blocking Peptide (#BLP-FT001).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of SLC19A1 in rat hippocampus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (1:1200), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA3 region, showed immunoreactivity (green) in neuronal profiles in the pyramidal layer (arrows). B. Pre-incubation of the Antibody with SLC19A1/RFC1 (extracellular) Blocking Peptide (BLP-FT001), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of SLC19A1 in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (5 µg) + goat- Anti-rabbit-FITC.
