MA5-18292

antibody from Invitrogen Antibodies

Targeting: ARID5A MRF-1, RP11-363D14

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-18292

Provider

Invitrogen Antibodies

Product name

ARID5A Monoclonal Antibody (P18112)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 3xFlag-human ARID5A-transfected 293T, THP-1, THP-1 nuclear extract, Raw264.7, Raw264.7 (50 ng/mL LPS, 4hr).

Antibody clone number

P18112

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ARID5A was performed by separating 30 µg of THP-1 whole cell and nuclear extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ARID5A Monoclonal Antibody (P18112) (Product # MA5-18292) at a dilution of 1:500The signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ARID5A Polyclonal Antibody detects ARID5A protein by western blot analysis. Non-transfected (-) and ARID5A-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A Polyclonal Antibody ARID5A Monoclonal Antibody (P18112) (Product # MA5-18292) diluted by 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ARID5A was performed by separating 30 µg of untreated (–) and treated (+) Raw264.7 whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ARID5A Monoclonal Antibody (P18112) (Product # MA5-18292) at a dilution of 1:500. (LPS: Lipopolysaccharides).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Frozen) analysis of ARID5A was performed in frozen-sectioned mouse mouse cerebellum tissue using ARID5A Monoclonal Antibody (P18112) (Product # MA5-18292) at a dilution of 1:250 (Green). Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of ARID5A was performed in THP-1 cells using ARID5A Monoclonal Antibody (P18112) (Product # MA5-18292) (red) at a dilution of 1:20. Black: Unlabelled sample was used as a control. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.