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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-18306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARID5A Monoclonal Antibody (P921)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 3xFlag-human ARID5A-transfected 293T, THP-1 nuclear extract.
- Antibody clone number
- P921
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ARID5A was performed by separating 30 µg of THP-1 whole cell and nuclear extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ARID5A Monoclonal Antibody (P921) (Product # MA5-18306) at a dilution of 1:500The signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID5A Polyclonal Antibody detects ARID5A protein by western blot analysis. Non-transfected (-) and ARID5A-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A Polyclonal Antibody ARID5A Monoclonal Antibody (P921) (Product # MA5-18306) diluted by 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of ARID5A was performed in THP-1 cells using ARID5A Monoclonal Antibody (P921) (Product # MA5-18306) (red) at a dilution of 1:20. Black: Unlabelled sample was used as a control. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
