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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-43745 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ARID5A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARID5A Antibody (18112) [NBP2-43745] - Analysis of Non-transfected (-) and ARID5A-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody diluted by 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARID5A Antibody (18112) [NBP2-43745] - Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody 18112]. The signal was developed with Trident ECL plus-Enhanced. (LPS: Lipopolysaccharides)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARID5A Antibody (18112) [NBP2-43745] - THP-1 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [18112] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: ARID5A Antibody (18112) [NBP2-43745] - Sectioned mouse mouse cerebellum. Green: ARID5A stained by ARID5A antibody [18112] diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ARID5A Antibody (18112) [NBP2-43745] - ARID5A antibody [18112] detects ARID5A protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: ARID5A antibody [18112] dilution: 1:20. Acquisition of 20,000 events were collected using a DyLight 488-conjugated secondary antibody for FACS analysis.
