19887-1-AP

antibody from Proteintech Group

Targeting: ILF3 DRBP76, MPHOSPH4, MPP4, MPP4110, NF110, NF110b, NF90, NF90a, NF90c, NF90ctv, NFAR-1, NFAR-2, NFAR110, NFAR90, TCP110

Provider product page for 19887-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [26]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 19887-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#19887-1-AP, RRID:AB_10666431
Product name: ILF3 antibody
Antibody type: Polyclonal
Description: KD/KO validated ILF3 antibody (Cat. #19887-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ILF3 protein structure - 19887-1-AP shown in red.

Submitted references A therapeutically targetable positive feedback loop between lnc-HLX-2-7, HLX, and MYC that promotes group 3 medulloblastoma.

Katsushima K, Joshi K, Yuan M, Romero B, Batish M, Stapleton S, Jallo G, Kolanthai E, Seal S, Saulnier O, Taylor MD, Wechsler-Reya RJ, Eberhart CG, Perera RJ
Cell reports 2024 Mar 26;43(3):113938

A mutation in the low-complexity domain of splicing factor hnRNPA1 linked to amyotrophic lateral sclerosis disrupts distinct neuronal RNA splicing networks.

Lee YJ, Rio DC
Genes & development 2024 Feb 13;38(1-2):11-30

Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape.

Cao Y, Wu J, Hu Y, Chai Y, Song J, Duan J, Zhang S, Xu X
Cell reports 2023 Oct 31;42(10):113262

Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing.

Yan G, Yang J, Li W, Guo A, Guan J, Liu Y
Nature cell biology 2023 May;25(5):754-764

ILF3 prion-like domain regulates gene expression and fear memory under chronic stress.

Yamashita A, Shichino Y, Fujii K, Koshidaka Y, Adachi M, Sasagawa E, Mito M, Nakagawa S, Iwasaki S, Takao K, Shiina N
iScience 2023 Mar 17;26(3):106229

Astragaloside IV promotes keratinocyte proliferation and migration through upregulating lncRNA H19 recruited ILF3 to enhance the stability of CDK4 mRNA.

Wang DD, Zhang LZ, Pang CJ, Ye JZ
The Kaohsiung journal of medical sciences 2023 Aug;39(8):811-823

Lipopolysaccharide induces placental mitochondrial dysfunction in murine and human systems by reducing MNRR1 levels via a TLR4-independent pathway.

Purandare N, Kunji Y, Xi Y, Romero R, Gomez-Lopez N, Fribley A, Grossman LI, Aras S
iScience 2022 Nov 18;25(11):105342

LncRNA ELF3-AS1 inhibits gastric cancer by forming a negative feedback loop with SNAI2 and regulates ELF3 mRNA stability via interacting with ILF2/ILF3 complex.

Li D, Shen L, Zhang X, Chen Z, Huang P, Huang C, Qin S
Journal of experimental & clinical cancer research : CR 2022 Dec 2;41(1):332

ILF3 represses repeat-derived microRNAs targeting RIG-I mediated type I interferon response.

Chen G, Yang Y, Wu QJ, Cao L, Ruan W, Shao C, Jiang L, Tang P, Ma S, Jiang A, Wang Z, Wu K, Zhang QC, Fu XD, Zhou Y
Journal of molecular biology 2022 Apr 15;434(7):167469

miRNA-mediated loss of m6A increases nascent translation in glioblastoma.

Zepecki JP, Karambizi D, Fajardo JE, Snyder KM, Guetta-Terrier C, Tang OY, Chen JS, Sarkar A, Fiser A, Toms SA, Tapinos N
PLoS genetics 2021 Mar;17(3):e1009086

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Ma Y, Liu S, Gao J, Chen C, Zhang X, Yuan H, Chen Z, Yin X, Sun C, Mao Y, Zhou F, Shao Y, Liu Q, Xu J, Cheng L, Yu D, Li P, Yi P, He J, Geng G, Guo Q, Si Y, Zhao H, Li H, Banes GL, Liu H, Nakamura Y, Kurita R, Huang Y, Wang X, Wang F, Fang G, Engel JD, Shi L, Zhang YE, Yu J
Developmental cell 2021 Feb 22;56(4):478-493.e11

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Sun L, Li P, Ju X, Rao J, Huang W, Ren L, Zhang S, Xiong T, Xu K, Zhou X, Gong M, Miska E, Ding Q, Wang J, Zhang QC
Cell 2021 Apr 1;184(7):1865-1883.e20

Aberrant USP11 expression regulates NF90 to promote proliferation and metastasis in hepatocellular carcinoma.

Zhang C, Xie C, Wang X, Huang Y, Gao S, Lu J, Lu Y, Zhang S
American journal of cancer research 2020;10(5):1416-1428

CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA-Binding Proteins and Sponging MiR-942.

Wong CH, Lou UK, Li Y, Chan SL, Tong JH, To KF, Chen Y
Cancer research 2020 Jun 1;80(11):2138-2149

Long non-coding RNA ELDR enhances oral cancer growth by promoting ILF3-cyclin E1 signaling.

Sur S, Nakanishi H, Steele R, Zhang D, Varvares MA, Ray RB
EMBO reports 2020 Dec 3;21(12):e51042

Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.

Xu S, Zhan M, Jiang C, He M, Yang L, Shen H, Huang S, Huang X, Lin R, Shi Y, Liu Q, Chen W, Mohan M, Wang J
Nature communications 2019 Dec 2;10(1):5492

Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.

Azam S, Hou S, Zhu B, Wang W, Hao T, Bu X, Khan M, Lei H
RNA biology 2019 Aug;16(8):1001-1009

PDGFRβ translocates to the nucleus and regulates chromatin remodeling via TATA element-modifying factor 1.

Papadopoulos N, Lennartsson J, Heldin CH
The Journal of cell biology 2018 May 7;217(5):1701-1717

EGFR-mediated interleukin enhancer-binding factor 3 contributes to formation and survival of cancer stem-like tumorspheres as a therapeutic target against EGFR-positive non-small cell lung cancer.

Cheng CC, Chou KF, Wu CW, Su NW, Peng CL, Su YW, Chang J, Ho AS, Lin HC, Chen CG, Yang BL, Chang YC, Chiang YW, Lim KH, Chang YF
Lung cancer (Amsterdam, Netherlands) 2018 Feb;116:80-89

The cellular stress proteins CHCHD10 and MNRR1 (CHCHD2): Partners in mitochondrial and nuclear function and dysfunction.

Purandare N, Somayajulu M, Hüttemann M, Grossman LI, Aras S
The Journal of biological chemistry 2018 Apr 27;293(17):6517-6529

Interleukin-2 enhancer binding factor 2 interacts with the nsp9 or nsp2 of porcine reproductive and respiratory syndrome virus and exerts negatively regulatory effect on the viral replication.

Wen X, Bian T, Zhang Z, Zhou L, Ge X, Han J, Guo X, Yang H, Yu K
Virology journal 2017 Jul 11;14(1):125

Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration.

Aras S, Arrabi H, Purandare N, Hüttemann M, Kamholz J, Züchner S, Grossman LI
Biochimica et biophysica acta. Molecular cell research 2017 Feb;1864(2):440-448

Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma.

Zhang LF, Lou JT, Lu MH, Gao C, Zhao S, Li B, Liang S, Li Y, Li D, Liu MF
The EMBO journal 2015 Nov 3;34(21):2671-85

MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism.

Aras S, Bai M, Lee I, Springett R, Hüttemann M, Grossman LI
Mitochondrion 2015 Jan;20:43-51

Expression of leukemia inhibitory factor in Müller glia cells is regulated by a redox-dependent mRNA stability mechanism.

Agca C, Boldt K, Gubler A, Meneau I, Corpet A, Samardzija M, Stucki M, Ueffing M, Grimm C
BMC biology 2015 Apr 25;13:30

Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2.

Aras S, Pak O, Sommer N, Finley R Jr, Hüttemann M, Weissmann N, Grossman LI
Nucleic acids research 2013 Feb 1;41(4):2255-66

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 19887-1-AP(NF90,ILF3 antibody) at dilution of 1:800
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using ILF3 antibody 19887-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-NF90,ILF3 (IP:19887-1-AP, 3ug; Detection:19887-1-AP 1:1000) with HeLa cells lysate 1600ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human cervical cancer using 19887-1-AP(NF90,ILF3 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue