A303-122A

antibody from Invitrogen Antibodies

Targeting: ILF3 DRBP76, MPHOSPH4, MPP4, MPP4110, NF110, NF110b, NF90, NF90a, NF90c, NF90ctv, NFAR-1, NFAR-2, NFAR110, NFAR90, TCP110

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A303-122A

Provider

Invitrogen Antibodies

Product name

NF110 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NF110 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF110 antibody (Product # A303-122A; lot A303-122A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse NF110 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF110 antibody (Product # A303-122A; lot A303-122A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NF110 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF110 antibody A303-122A (lot A303-122A-2) used for IP at 6 µg per reaction. NF110 was also immunoprecipitated by a previous lot of this antibody A303-122A-1. For blotting immunoprecipitated NF110, A303-122A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse NF110 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF110 antibody A303-122A (lot A303-122A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NF110 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NF110 (Product # A303-122A; lot 3) used at a dilution of 1:5,000 (0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NF110 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- NF110 (Cat. No. A303-122A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NF110 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF110 antibody (Product # A303-122A; lot A303-122A-3) used for IP at 6 µg per reaction. NF110 was also immunoprecipitated by a previous lot of this antibody (lot A303-122A-2). For blotting immunoprecipitated NF110 (Product # A303-122A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.