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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA1-32315 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-DNMT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNMT1 in 50µ of total protein lysate from untreated T24 cells (lane 1) and 50 µg of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days (lane 2), using a DNMT1 polyclonal antibody (Product # PA1-32315).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DNMT1 was performed by loading HEK293 cell lysate. Proteins were transferred to a membrane and probed with a DNMT1 Polyclonal Antibody (Product # PA1-32315).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DNMT1 in HeLa cells using a DNMT1 polyclonal antibody (Product # PA1-32315) at a dilution of 1:50 against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
