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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB7543 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7543, RRID:AB_1574427
- Product name
- DNMT1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of DNMT1.
- Antigen sequence
C-RFESPPKTQPTEDN- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline.
Dion V, Lin Y, Hubert L Jr, Waterland RA, Wilson JH
Human molecular genetics 2008 May 1;17(9):1306-17
Human molecular genetics 2008 May 1;17(9):1306-17
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DNMT1 polyclonal antibody (Cat # PAB7543) (0.5 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DNMT1 polyclonal antibody (Cat # PAB7543)(3.8 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
