Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- A00172-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Dnmt1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for DNMT1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC/IF, Direct ELISA. Reactive species: Human. DNMT1 information: Subcellular Localization: Nucleus; Tissue Specificity: Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Folic acid intervention changes liver Foxp3 methylation and ameliorates the damage caused by Th17/Treg imbalance after long-term alcohol exposure.
Thioguanine Induces Apoptosis in Triple-Negative Breast Cancer by Regulating PI3K-AKT Pathway.
Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer's Disease-Like Features in Rats.
Circular RNA CpG island hypermethylation-associated silencing in human cancer.
DNMT1-mediated PTEN hypermethylation confers hepatic stellate cell activation and liver fibrogenesis in rats.
Zhao H, Guo P, Zuo Y, Wang Y, Zhao H, Lan T, Xue M, Zhang H, Liang H
Food & function 2022 May 10;13(9):5262-5274
Food & function 2022 May 10;13(9):5262-5274
Thioguanine Induces Apoptosis in Triple-Negative Breast Cancer by Regulating PI3K-AKT Pathway.
Zhang D, An X, Li Q, Man X, Chu M, Li H, Zhang N, Dai X, Yu H, Li Z
Frontiers in oncology 2020;10:524922
Frontiers in oncology 2020;10:524922
Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer's Disease-Like Features in Rats.
Zeng P, Shi Y, Wang XM, Lin L, Du YJ, Tang N, Wang Q, Fang YY, Wang JZ, Zhou XW, Lu Y, Tian Q
The international journal of neuropsychopharmacology 2019 Jan 1;22(1):57-70
The international journal of neuropsychopharmacology 2019 Jan 1;22(1):57-70
Circular RNA CpG island hypermethylation-associated silencing in human cancer.
Ferreira HJ, Davalos V, de Moura MC, Soler M, Perez-Salvia M, Bueno-Costa A, Setien F, Moran S, Villanueva A, Esteller M
Oncotarget 2018 Jun 26;9(49):29208-29219
Oncotarget 2018 Jun 26;9(49):29208-29219
DNMT1-mediated PTEN hypermethylation confers hepatic stellate cell activation and liver fibrogenesis in rats.
Bian EB, Huang C, Ma TT, Tao H, Zhang H, Cheng C, Lv XW, Li J
Toxicology and applied pharmacology 2012 Oct 1;264(1):13-22
Toxicology and applied pharmacology 2012 Oct 1;264(1):13-22
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Dnmt1 using anti-Dnmt1 antibody (A00172-1). Dnmt1 was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Dnmt1 Antibody (A00172-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image