Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-7002-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Dnmt1 Antibody
- Antibody type
- Monoclonal
- Description
- Dnmt1(DNA (cytosine-5-)-methyltransferase 1 ) is one of the most abundant DNA methyltransferase in mammalian tissues, where it associates with the replication machinery and restores symmetrical methylation at hemimethylated CpG sites generated by the semi-conservative DNA replication process. Dnmt1 comprises a regulatory N-terminal region and a C-terminal catalytic domain connected by a linker of seven glycine-lysine repeats. The N-terminal part contains a PCNA binding domain (PBD), a heterochromatin targeting sequence (TS), a CXXC-type zinc finger domain and two Bromo-Adjacent Homology domains (BAH1 and BAH2). The C-terminal domains of mammalian Dnmts contain all ten catalytic motifs identified in bacterial DNA (cytosine-5) methyltransferases. Dnmt1 maintains methylation patterns with high fidelity and is essential for embryonic development and genome integrity. The molecules interacting with Dnmt1, including RNA polymerase II, some RNA-binding proteins, and some specific Dnmt1-inhibitory RNA molecules are involved in chromatin organization, DNA repair, cell cycle regulation, and apoptosis.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant DNMT1
protein (amino acids 128-429) was u
sed as the immunogen for this antib
ody.- Isotype
- IgG
- Antibody clone number
- ABM13B2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of DNMT1. Anti- DNMT1 antibody (Clone: ABM13B2) was used at 2 µg/ml on mouse Embryonic liver lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dnmt1 in human SKM tissue using Dnmt 1 antibody (Clone: ABM13B2) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dnmt1 in human Placenta tissue using Dnmt 1 antibody (Clone: ABM13B2) at 5 µg/ml.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flow analysis of Dnmt1 in Hek-293 cells using 0.5 µg/10^6 cells of Dnmt1 antibody (Clone: ABM13B2). Green represents isotype control; red represents anti-DNMT1 antibody. Goat anti-Mouse PE conjugate was used as secondary antibody.
- Protocol
- Protocol