Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- PAB14077 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14077, RRID:AB_10550289
- Product name
- Dnmt1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Dnmt1.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot was performed on whole cell lysates from mouse fibroblasts (Lane 1, NIH/3T3) and embryonic stem cells (Lane 2, E14Tg2a) with Dnmt1 polyclonal antibody (Cat # PAB14077), diluted 1 : 500 in BSA/PBS-Tween.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of Dnmt1 polyclonal antibody (Cat # PAB14077).The plates were coated with the peptide used for immunization of the rabbit.By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 9,600.
- Validation comment
- ELISA