Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB15590 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB15590, RRID:AB_10678356
- Product name
- Dnmt1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant Dnmt1.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Epigenetics of eu- and heterochromatin in inverted and conventional nuclei from mouse retina.
The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA.
Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor.
DNMT1 and DNMT3b cooperate to silence genes in human cancer cells.
Eberhart A, Feodorova Y, Song C, Wanner G, Kiseleva E, Furukawa T, Kimura H, Schotta G, Leonhardt H, Joffe B, Solovei I
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2013 Aug;21(5):535-54
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2013 Aug;21(5):535-54
The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA.
Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, Endo TA, Shinga J, Mizutani-Koseki Y, Toyoda T, Okamura K, Tajima S, Mitsuya K, Okano M, Koseki H
Nature 2007 Dec 6;450(7171):908-12
Nature 2007 Dec 6;450(7171):908-12
Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor.
Di Croce L, Raker VA, Corsaro M, Fazi F, Fanelli M, Faretta M, Fuks F, Lo Coco F, Kouzarides T, Nervi C, Minucci S, Pelicci PG
Science (New York, N.Y.) 2002 Feb 8;295(5557):1079-82
Science (New York, N.Y.) 2002 Feb 8;295(5557):1079-82
DNMT1 and DNMT3b cooperate to silence genes in human cancer cells.
Rhee I, Bachman KE, Park BH, Jair KW, Yen RW, Schuebel KE, Cui H, Feinberg AP, Lengauer C, Kinzler KW, Baylin SB, Vogelstein B
Nature 2002 Apr 4;416(6880):552-6
Nature 2002 Apr 4;416(6880):552-6
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts.Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene.Biotylated Np95 was captured by streptoavidin beads.The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 and Pcna.( The data were kindly provided by Dr. M. Muto of Riken Research Center for Allergy and Immunology ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Catalytic activity of NP95 associated Dnmt1 in HeLa cells.The presence of Dnmt1 in the NP95 complexes was confirmed by western blot and the DNA methyltransferase activity of the extracts was measured (The data were kindly provided by Dr. M. Muto of Riken Research Center for Allergy and Immunology).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Subnuclear localization of Dnmt1 (green), Np95 (red) and Pcna (blue) in mouse embryonic stem cells (E14) during cell-cycle progression (bottom).Merged images for Np95 and Dnmt1 are shown. Profile of DNA content in exponentially growing ECSs is shown (top).(The data were kindly provided by Dr. M. Muto of Riken Research Center for Allergy and Immunology).
- Validation comment
- Immunofluorescence
