Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [2]
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Validation data
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- Product number
- NB100-392 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-392, RRID:AB_10003123
- Product name
- Rabbit Polyclonal DNMT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Epigenetic regulation of motor neuron cell death through DNA methylation.
Chestnut BA, Chang Q, Price A, Lesuisse C, Wong M, Martin LJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Nov 16;31(46):16619-36
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Nov 16;31(46):16619-36
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT1 Antibody [NB100-392] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT1 Antibody [NB100-392] - Western blot for Dnmt1 in mouse brain cells. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody NB100-392 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 in HeLa nuclear extracts [50 ug/lane (A), 10 ug input, or 1 mg NB 100-392 IP (B)] using NB 100-392. DNMT1 immunoprecipitated with NB 100-392 and detected with NB 100-392 using 0.2 ug/ml. 2 second ECL exposure.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody NB100-392 used for IP at 3 ug per reaction. For blotting immunoprecipitated DNMT1, NB100-392 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.