Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NB100-391 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-391, RRID:AB_10001957
- Product name
- Rabbit Polyclonal DNMT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Selective death of cancer cells by preferential induction of reactive oxygen species in response to (-)-epigallocatechin-3-gallate.
Min NY, Kim JH, Choi JH, Liang W, Ko YJ, Rhee S, Bang H, Ham SW, Park AJ, Lee KH
Biochemical and biophysical research communications 2012 Apr 27;421(1):91-7
Biochemical and biophysical research communications 2012 Apr 27;421(1):91-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT1 Antibody [NB100-391] - Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT1 Antibody [NB100-391] - Detection of human DNMT1 in HeLa nuclear extracts [50 ug/lane (A), 10 ug input, or 1 mg NB 100-392 IP (B)] using NB 100-391. DNMT1 immunoprecipitated with NB 100-392 and detected with NB 100-391 using 0.2 ug/ml. 2 second ECL exposure.